Regulated systemic activation of rat plasma prorenin.
Regulated systemic activation of rat plasma prorenin.
复制标题
调节大鼠血浆肾素原的全身激活。
DOI：
10.1161/01.hyp.10.1.122
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发表时间：
1987
期刊：
影响因子：
8.3
通讯作者：
D. Osmond
中科院分区：
文献类型：
--
作者：
G. M. Hare;P. Ioannou;S. Dubiski;D. Osmond
肾切除大鼠血浆原肾素水平高于正常水平，推测是肾外来源，但基本上没有肾素，表明缺乏原肾素活化的“转化酶”。肾上腺切除大鼠血浆原肾素水平低，伴随着高肾素活性，这表明增强的原肾素激活的作用，刺激的“转化酶”机制。肾上腺切除和肾切除大鼠之间的交叉循环15或30分钟，显着降低原肾素和提高在这两种类型的大鼠的肾素水平，表明广泛激活原肾素的肾素。同样，在体外混合这些血液（没有交叉循环）提高了五倍以上的预期计算水平的肾素活性，而原肾素基本上消失了。在这两种情况下，从肾切除大鼠血浆中的原肾素显然被激活的增强作用的“转化酶”在肾上腺切除大鼠血浆中的肾素。这种新产生的血管紧张素活性与正常血浆血管紧张素一样，几乎完全被抗猪血管紧张素的单克隆抗体抑制，并产生免疫反应性血管紧张素I。相反，交叉循环或体外混合正常对照组和肾切除大鼠的血液产生很少可检测到的激活的原肾素和只有适度的增量的肾素，这表明在正常血浆中的“转化酶”机制的相对不活跃。我们的数据表明，血浆原肾素的激活是一个重要的调节途径，为肾素生产，因为它是大大刺激后肾上腺切除术和肾切除术后缺乏，从而牵连肾脏作为一个重要的贡献者的“转化酶”机制内循环操作。
Nephrectomized rats have above-normal plasma prorenin levels, presumably of extra-renal origin, but essentially no renin, suggesting a lack of "convertase" for prorenin activation. Adrenalectomized rats have low plasma prorenin levels accompanied by high renin activity, suggesting enhanced prorenin activation by the action of a stimulated "convertase" mechanism. Cross-circulation between adrenalectomized and nephrectomized rats for 15 or 30 minutes, dramatically lowered prorenin and raised renin levels in both types of rats, suggesting extensive activation of prorenin to renin. Similarly, in vitro mixing of these bloods (without cross-circulation) raised renin activity over five times the expected calculated level, while prorenin essentially disappeared. In both cases, prorenin from nephrectomized rat plasma apparently was activated to renin by the enhanced action of "convertase" in the adrenalectomized rat plasma. This newly generated renin activity was, like normal plasma renin, almost completely inhibited by a monoclonal antibody against hog renin and generated an immunoreactive angiotensin I. In contrast, cross-circulation or in vitro mixing of blood from normal control and nephrectomized rats produced little detectable activation of prorenin and only modest increments of renin, suggesting relative inactivity of the "convertase" mechanism in normal plasma. Our data suggest that activation of plasma prorenin is a significant regulated pathway for renin production, as it is greatly stimulated after adrenalectomy and deficient after nephrectomy, thereby implicating the kidney as an important contributor to the "convertase" mechanism operating within the circulation.
肾素原和其他大分子量形式的肾素。
影响因子：
20.3
作者：
Sealey,JE;Atlas,SA;Laragh,JH
通讯作者：
Laragh,JH
狗失活肾素：生化特征和分泌到肾血浆和淋巴液中。
DOI：
10.1152/ajpendo.1986.250.1.e55
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
--
作者：
Dzau,VJ;Wilcox,CS;Sands,K;Dunckel,P
通讯作者：
Dunckel,P