Aggregation of a parthenogenetic diploid embryo and a male embryo improves the blastocyst development and parthenogenetic embryonic stem cell derivation
Aggregation of a parthenogenetic diploid embryo and a male embryo improves the blastocyst development and parthenogenetic embryonic stem cell derivation
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孤雌二倍体胚胎和雄性胚胎的聚集改善囊胚发育和孤雌胚胎干细胞衍生
DOI：
10.3906/biy-1612-30
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发表时间：
2017-08
影响因子：
2.2
通讯作者：
Yuemin LI
中科院分区：
文献类型：
--
作者：
Xiaoyan QIU;Nan LI;Xiong XIAO;Yuemin LI
无父本基因组的孤雌生殖哺乳动物胚胎不能存活并死亡，主要是由于缺乏父本效应导致的胎盘生长缺陷，导致孤雌生殖胚胎干细胞（pESC）的囊胚形成率低和衍生效率低。因此，本研究通过优化小鼠孤雌胚胎的生产工艺，将孤雌二倍体胚胎与已鉴定的雄性胚胎进行融合，旨在探索一种提高小鼠孤雌胚胎发育和pESC衍生的方法。采用不同的化学组合进行优化，发现杂合二倍体型的囊胚形成率显著高于单倍体型（P < 0.05）。氯化锶（SrCl 2）与细胞松弛素B联合处理4 h，杂合二倍体率和囊胚形成率最高。利用自制的凹孔系统将孤雌生殖杂合二倍体胚胎与经双重PCR鉴定的雄性胚胎进行融合，发现融合胚胎的囊胚形成率和pESC分离率均有所提高。
Parthenogenetically derived mammalian embryos, with no paternal genome, are not viable and die, largely from defective placental growth attributed to a lack of paternal effect, resulting in the low blastulation rate and low derivation efficiency of parthenogenetic embryonic stem cells (pESCs). Therefore, the present study, by the optimization of parthenogenetic embryo production and the aggregation of the parthenogenetic diploid embryo and the identified male embryo, aims to investigate a method to improve the development of parthenogenetic embryo and pESC derivation in mice. Using different chemical combinations for the optimization, we found that the heterozygous diploid type had a significantly higher blastulation rate than the haploid type (P < 0.05). The treatment of strontium chloride (SrCl2) combined with cytochalasin B for 4 h produced the highest heterozygous diploid rate and blastulation rate. Our self-made concave hole system was used for the aggregation of the parthenogenetic heterozygous diploid embryo with the male embryo identified by the duplex PCR method, and we found that the chimeric embryo had an improved rate of blastulation and pESC isolation.
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影响因子：
4.6
作者：
P. Szabó;J. Mann
通讯作者：
P. Szabó;J. Mann
影响因子：
4.6
作者：
Zhi-Yan Shan;Yanshuang Wu;Xing-hui Shen;Xue Li;Yuan Xue;Zhong Zheng;Zhen-dong Wang;Chun-Jia Liu;Ruizhen Sun;Zhao-yuan Li;Jing-ling Shen;Zhong-hua Liu;Lei Lei-Lei
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Zhi-Yan Shan;Yanshuang Wu;Xing-hui Shen;Xue Li;Yuan Xue;Zhong Zheng;Zhen-dong Wang;Chun-Jia Liu;Ruizhen Sun;Zhao-yuan Li;Jing-ling Shen;Zhong-hua Liu;Lei Lei-Lei
影响因子：
2.6
作者：
C. Walsh;A. Glaser;R. Fundele;A. Ferguson-Smith;S. Barton;M. Surani;R. Ohlsson
通讯作者：
C. Walsh;A. Glaser;R. Fundele;A. Ferguson-Smith;S. Barton;M. Surani;R. Ohlsson
影响因子：
2.7
作者：
R. Moses;Douglas Kline
通讯作者：
R. Moses;Douglas Kline
影响因子：
2.8
作者：
Ma, SF;Liu, XY;Tan, JH
通讯作者：
Tan, JH