Phosphatidylinositol phosphate 5-kinase genes respond to phosphate deficiency for root hair elongation in Arabidopsis thaliana.
Phosphatidylinositol phosphate 5-kinase genes respond to phosphate deficiency for root hair elongation in Arabidopsis thaliana.
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拟南芥中磷脂酰肌醇磷酸 5 激酶基因对磷酸盐缺乏作出反应，导致根毛伸长。
DOI：
10.1111/tpj.12741
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Wada;Y.;Kusano;H.;Tsuge;T.;and Aoyama;T.
植物急剧改变其根系结构，以适应不同的地下生长条件。在磷缺乏时，包括拟南芥在内的大多数植物的侧根和根毛的发育增强，导致丛生根和毛状根。为了阐明根毛伸长对磷缺乏反应的特异性信号通路，我们研究了B型磷脂酰肌醇磷酸5-激酶（PIP 5 K）基因的表达，作为拟南芥根毛伸长的定量因子。在幼苗阶段，PIP 5 K3和PIP 5 K4基因通过其上游区域的PHR 1结合序列（P1 BS）在稳态转录水平上响应于Pi缺乏。pip 5 k3和pip 5 k4单突变体表现为短根毛表型，对缺磷仍有根毛伸长的响应;而pip 5 k3和pip 5 k4双突变体表现为短根毛，在幼苗期对缺磷失去响应。在策略互补系中，其中修饰的PIP 5 K3和PIP 5 K4基因在其P1 BS中具有碱基取代，被共同引入双突变体中，幼苗的根毛在Pi充足的条件下具有正常长度，但对Pi缺乏没有反应。根据这些结果，我们得出结论，Pi缺乏信号通过PIP 5 K基因转移到根毛伸长的途径。
Plants drastically alter their root system architecture to adapt to different underground growth conditions. During phosphate (Pi) deficiency, most plants includingArabidopsis thalianaenhance the development of lateral roots and root hairs, resulting in bushy and hairy roots. To elucidate the signal pathway specific for the root hair elongation response to Pi deficiency, we investigated the expression of type‐B phosphatidylinositol phosphate 5‐kinase (PIP5K) genes, as a quantitative factor for root hair elongation in Arabidopsis. At young seedling stages, thePIP5K3andPIP5K4genes responded to Pi deficiency in steady‐state transcript levels via PHR1‐binding sequences (P1BSs) in their upstream regions. Bothpip5k3andpip5k4single mutants, which exhibit short‐root‐hair phenotypes, remained responsive to Pi deficiency for root hair elongation; however thepip5k3pip5k4double mutant exhibited shorter root hairs than the single mutants, and lost responsiveness to Pi deficiency at young seedling stages. In the tactical complementation line in which modifiedPIP5K3andPIP5K4genes with base substitutions in their P1BSs were co‐introduced into the double mutant, root hairs of young seedlings had normal lengths under Pi‐sufficient conditions, but were not responsive to Pi deficiency. From these results, we conclude that a Pi‐deficiency signal is transferred to the pathway for root hair elongation via the PIP5K genes.
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DOI：
10.1073/pnas.0500778102
发表时间：
2005-05-24
影响因子：
11.1
作者：
Miura, K;Rus, A;Hasegawa, PM
通讯作者：
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磷酸盐的可用性改变了水曲柳的侧根解剖结构和根结构。
DOI：
--
发表时间：
2005
期刊：
影响因子：
--
作者：
Chu Wu;Xing Wei;Hai;Zhengquan Wang
通讯作者：
Zhengquan Wang
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DOI：
10.1042/bj20060565
发表时间：
2006
期刊：
The Biochemical journal.
影响因子：
--
作者：
Santarius,Megan;Lee,ChangHo;Anderson,RichardA
通讯作者：
Anderson,RichardA