Diagnostic accuracy of DNA methylation in detection of gastric cancer: a meta-analysis.
Diagnostic accuracy of DNA methylation in detection of gastric cancer: a meta-analysis.
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DNA 甲基化在胃癌检测中的诊断准确性：荟萃分析
DOI：
10.18632/oncotarget.22613
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发表时间：
2017-12-22
期刊：
影响因子：
--
通讯作者：
Si J
中科院分区：
文献类型：
--
作者：
Hu W;Zheng W;Liu Q;Chu H;Chen S;Kim JJ;Wu J;Si J
新兴的研究证明了基于DNA甲基化的血液检测对胃癌的诊断效用。荟萃分析的目的是评估血液DNA甲基化标志物检测胃癌患者的准确性。选择了一项截至2016年11月的系统性文献检索，评价了利用血液标本检测胃癌的DNA甲基化标志物，以获得汇总的灵敏度和特异性。包括4，172例患者（胃癌（N = 2，098），对照（N = 2，074））的32项研究符合研究标准。基于DNA甲基化的血液检测检测胃癌的总体灵敏度为57%（95%CI 50-63%）;特异性为97%（95%CI 95-98%）。在接受血浆检测的患者中，灵敏度为71%（95% CI 59-81%）;特异性为89%（95% CI 78-94%）。在接受血清检测的患者中，灵敏度为50%（95% CI 43-58%）;特异性为98%（95% CI 96-99%）。使用多个甲基化基因的敏感性为76%（95%CI 64-84%）;特异性为85%（95%CI 65-95%）。DNA甲基化检测对TNM I+II期胃癌的敏感性为55%（95%CI 47-64%），特异性为96%（95%CI 92-98%）。结论：血液DNA甲基化检测对胃癌的诊断具有较高的特异性，但敏感性不高。检测多个甲基化基因或使用血浆样本可能会提高诊断的敏感性。
Emerging studies demonstrate the diagnostic utility of DNA methylation-based blood test for gastric cancer. The aim of the meta-analysis is to evaluate the accuracy of blood DNA methylation markers for detecting patients with gastric cancer. A systematic literature search to November 2016 that evaluated DNA methylation markers utilizing blood specimen to detect gastric cancer were selected to derive pooled sensitivities and specificities. 32 studies including 4,172 patients (gastric cancer (N = 2,098), control (N = 2,074)) met the study criteria. Overall sensitivity of DNA methylation-based blood test for detecting gastric cancer was 57% (95% CI 50–63%); specificity was 97% (95% CI 95–98%). Among patients who received plasma-based testing, sensitivity was 71% (95% CI 59–81%); specificity was 89% (95% CI 78–94%). Among patients who received serum-based testing, sensitivity was 50% (95% CI 43–58%); specificity was 98% (95% CI 96–99%). Using multiple methylated genes had sensitivity of 76% (95% CI 64–84%); specificity of 85% (95% CI 65–95%). DNA methylation test had sensitivity of 55% (95% CI 47–64%) and specificity of 96% (95% CI 92–98%) for detecting TNM stage I+II gastric cancer. In conclusion, blood-based DNA methylation test had high specificity but modest sensitivity for detecting gastric cancer. Evaluating multiple methylated genes or using plasma sample may improve the diagnostic sensitivity.
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DOI：
10.1073/pnas.93.18.9821
发表时间：
1996-09-03
影响因子：
11.1
作者：
Herman, JG;Graff, JR;Baylin, SB
通讯作者：
Baylin, SB
影响因子：
2.5
作者：
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Gazdar AF
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通讯作者：
Wei, Sheng
影响因子：
3.7
作者：
Huang YK;Yu JC;Kang WM;Ma ZQ;Ye X;Tian SB;Yan C
通讯作者：
Yan C
影响因子：
--
作者：
Liu, Lihua;Wang, Shan;Liu, Jianchao
通讯作者：
Liu, Jianchao