Driving the expression of the Salmonella enterica sv Typhimurium flagellum using flhDC from Escherichia coli results in key regulatory and cellular differences
Driving the expression of the Salmonella enterica sv Typhimurium flagellum using flhDC from Escherichia coli results in key regulatory and cellular differences
复制标题
使用来自大肠杆菌的 flhDC 驱动肠沙门氏菌 sv Typhimurium flagellum 的表达，导致关键的调控和细胞差异
DOI：
10.1101/333146
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Albanna A
中科院分区：
文献类型：
--
作者：
Albanna A
大肠埃希氏菌和肠沙门氏菌的鞭毛系统在遗传和功能上具有显著的同源性。这两个系统都由鞭毛特异的主调节器FlhD4C2控制。自从对鞭毛系统进行遗传分析的早期以来，人们就知道这一点。ColiflhDC可以补充∆的flhDC突变体INS。肠系膜。基因组革命已经确定了转录因子和/或DNA结合位点的遗传变化如何影响相关物种的表型结果。因此，我们有兴趣问：使用现代工具来询问鞭毛基因的表达和组装，替换flhDC编码序列INS会有什么影响。为你进场。Fhdc肠杆菌染色体基因座上的共生菌？我们表明，尽管所有创建的菌株都是能动的，但当hDCECare存在时，鞭毛基因的表达明显降低。这些变化可以归因于FlhD4C2DNA识别的影响和产生稳定的FlhD4C2复合体所需的蛋白质-蛋白质相互作用。此外，我们的数据表明。结肠内鞭毛微丝调节反馈环对鞭毛系统的输出有明显的差异。我们认为，在基于监管机构的异类表达做出假设时，需要进行尽职调查，即使是表现出显著同步性的系统，其行为方式也可能并不完全相同。
The flagellar systems ofEscherichia coliandSalmonella entericaexhibit a significant level of genetic and functional synteny. Both systems are controlled by the flagellar specific master regulator FlhD4C2. Since the early days of genetic analyses of flagellar systems it has been known thatE. coli flhDCcan complement a ∆flhDCmutant inS. enterica. The genomic revolution has identified how genetic changes to transcription factors and/or DNA binding sites can impact the phenotypic outcome across related species. We were therefore interested in asking: using modern tools to interrogate flagellar gene expression and assembly, what would the impact be of replacing theflhDCcoding sequences inS. entericafor theE. coligenes at theflhDC S. enterciachromosomal locus? We show that even though all strains created are motile, flagellar gene expression is measurably lower whenflhDCECare present. These changes can be attributed to the impact of FlhD4C2DNA recognition and the protein-protein interactions required to generate a stable FlhD4C2complex. Furthermore, our data suggests that inE. colithe internal flagellar FliT regulatory feedback loop has a marked difference with respect to output of the flagellar systems. We argue due diligence is required in making assumptions based on heterologous expression of regulators and that even systems showing significant synteny may not behave in exactly the same manner.
影响因子：
3.2
作者：
Claret, L;Hughes, C
通讯作者：
Hughes, C
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL