Dietary Mustard Seeds (Sinapis alba Linn) Suppress 1,2-Dimethylhydrazine-Induced Immuno-Imbalance and Colonic Carcinogenesis in Rats
Dietary Mustard Seeds (Sinapis alba Linn) Suppress 1,2-Dimethylhydrazine-Induced Immuno-Imbalance and Colonic Carcinogenesis in Rats
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膳食芥菜籽 (Sinapis alba Linn) 抑制 1,2-二甲基肼诱导的大鼠免疫失衡和结肠癌发生
DOI：
10.1080/01635581.2012.658948
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Yawei Lu
中科院分区：
文献类型：
--
作者：
Minggu Zhu;Hai;Wenling Guo;Xinyan Li;Lin Jin;U. Brunk;Jiahuai Han;Ming Zhao;Yawei Lu
在Wistar大鼠模型中，长期补充芥子（MS）的饮食显着改善诱导结直肠癌的1，2-二甲基肼（DMH）。发现脾细胞主要组织相容性复合物I类（MHCI）的表达显著增强，而主要组织相容性复合物II类（MHCII）的表达显著降低。与对照组相比，MS组脾脏B-和树突状细胞（DC）的比例增加。在DC细胞中MHCI和MHCII的表达增加，而在B细胞中，MHCI的表达增加，但MHCII的表达适度降低。MS组CD 8 + CD 28+和CD 4 + CD 28+细胞百分比升高，而CD 4 + CD 25 + Foxp 3+亚群降低。血浆分析表明，DMH暴露诱导白细胞介素（IL）-4，IL-5，IL-10和转化生长因子-β的扩增量，而MS喂养抵消了这种作用，但增强了IL-2，IL-12 p70，IL-21，TNF-α和干扰素-γ。在SW 480结肠腺癌细胞系中，来自MS喂养动物的脾T细胞的细胞毒性显著增加。在DMH暴露的大鼠中，脾脏T细胞中穿孔素的表达显著降低，而MS消除了这种抑制。总之，饮食MS抑制DMH诱导的免疫失衡以及大鼠结肠癌的发生。
In a Wistar rat model, prolonged supplementation of mustard seed (MS) to the diet significantly ameliorates the induction of colorectal carcinomas by 1,2-dimethylhydrazine (DMH). The expression of the splenocyte major histocompatibility complex class I (MHCI) was found significantly enhanced, whereas that of the major histocompatibility complex class II (MHCII) was significantly decreased. Compared to that of control animals, the proportion of spleenic B- and dendritic cells (DC) was amplified in the MS group. The expressions of MHCI, as well as that of MHCII, were increased in DC cells; whereas in B cells, MHCI expression was augmented but that of MHCII moderately decreased. The percentages of CD8+CD28+ and CD4+CD28+ cells were increased in the MS group, while the CD4+CD25+Foxp3+ subset was depressed. Plasma analysis showed that DMH-exposure induced amplified amounts of interleukin (IL)-4, IL-5, IL-10, and transforming growth factor-beta, whereas MS feeding counteracted this effect but enhanced IL-2, IL12p70, IL21, TNF-alpha, and interferon-gamma. In the SW480 colon adenocarcinoma cell-line, the cytotoxicity of spleenic T-cells from MS-fed animals was significantly increased. In the DMH-exposed rats, the expression of perforin in the spleenic T-cells was dramatically decreased, whereas MS abolished this depression. In summary, dietary MS suppresses DMH-induced immuno-imbalance as well as colon carcinogenesis in rats.
影响因子：
4.7
作者：
J. Vanamala;Tety Leonardi;B. Patil;S. Taddeo;M. Murphy;L. Pike;R. Chapkin;J. Lupton;N. Turner
通讯作者：
J. Vanamala;Tety Leonardi;B. Patil;S. Taddeo;M. Murphy;L. Pike;R. Chapkin;J. Lupton;N. Turner
全身转移的肿瘤反应性 T 淋巴细胞对肿瘤的浸润是抗肿瘤功效所必需的。
影响因子：
11.2
作者：
Mukai,S;Kjaergaard,J;Shu,S;Plautz,GE
通讯作者：
Plautz,GE
影响因子：
5.9
作者：
FREI, B
通讯作者：
FREI, B