Changes in the expression of transcription factors in pancreatic AR42J cells during differentiation into insulin-producing cells.
Changes in the expression of transcription factors in pancreatic AR42J cells during differentiation into insulin-producing cells.
复制标题
胰腺 AR42J 细胞分化为胰岛素产生细胞期间转录因子表达的变化。
作者：
Y. Zhang;H. Mashima;I. Kojima
胰腺AR42J细胞具有外分泌和神经内分泌特性，并在用激活素A和肝细胞生长因子（HGF）处理后转化为胰岛素产生细胞。我们研究了在分化过程中各种转录因子的mRNA表达的变化。在所研究的转录因子中，Pax4和neurogenin3的表达水平发生了显著变化。这两个因子在幼稚细胞中未检测到，而它们的mRNA水平在用激活素A和HGF处理后显著增加。因此，这两个因子是由激活素A诱导的。转染Pax4后，胰腺组织形态及胰腺多肽（PP）表达无明显变化。此外，反义Pax4的引入并不影响由激活素A和HGF诱导的向胰岛素产生细胞的转化。相反，神经生成素3的转染诱导的形态学变化类似于激活素A诱导的形态学变化。此外，转染neurogenin3可诱导PP的表达。相反，反义neurogenin 3的引入阻断了激活素A和HGF诱导的AR 42 J细胞的分化。这些结果表明，激活素A调节神经生成素3的表达，这对于AR 42 J分化为内分泌细胞至关重要。
Pancreatic AR42J cells possess both exocrine and neuroendocrine properties and convert to insulin-producing cells upon treatment with activin A and hepatocyte growth factor (HGF). We studied changes in the mRNA expression of various transcription factors during the course of differentiation. Among the transcription factors studied, expression levels of Pax4 and neurogenin3 changed significantly. These two factors were not detected in naive cells, whereas their mRNA levels were markedly increased after treatment with activin A and HGF. Thus, these two factors were induced by activin A. Transfection of Pax4 did not induce any changes in morphology or expression of pancreatic polypeptide (PP). Furthermore, introduction of antisense Pax4 did not affect the conversion into insulin-producing cells induced by activin A and HGF. In contrast, transfection of neurogenin3 induced morphological changes similar to those induced by activin A. In addition, transfection of neurogenin3 induced the expression of PP. Conversely, introduction of antisense neurogenin3 blocked the differentiation of AR42J cells induced by activin A and HGF. These results indicate that activin A regulates the expression of neurogenin3, which is critical for the differentiation of AR42J into endocrine cells.
影响因子：
4.6
作者：
M. F. Offield;T. Jetton;P. Labosky;M. Ray;R. Stein;M. Magnuson;B. Hogan;C. Wright
通讯作者：
M. F. Offield;T. Jetton;P. Labosky;M. Ray;R. Stein;M. Magnuson;B. Hogan;C. Wright
影响因子：
10.5
作者：
Naya, FJ;Huang, HP;Tsai, MJ
通讯作者：
Tsai, MJ
胰岛发育的细胞谱系分析：胰高血糖素和胰岛素细胞源自胰管中存在的儿茶酚胺能前体。
影响因子：
2.7
作者：
Teitelman,G;Lee,JK
通讯作者：
Lee,JK