Cell lineage analysis of pancreatic islet development: glucagon and insulin cells arise from catecholaminergic precursors present in the pancreatic duct.
Cell lineage analysis of pancreatic islet development: glucagon and insulin cells arise from catecholaminergic precursors present in the pancreatic duct.
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胰岛发育的细胞谱系分析：胰高血糖素和胰岛素细胞源自胰管中存在的儿茶酚胺能前体。
DOI：
10.1016/0012-1606(87)90182-5
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发表时间：
1987
影响因子：
2.7
通讯作者：
Lee,JK
中科院分区：
文献类型：
--
作者：
Teitelman,G;Lee,JK
我们以前曾报道，细胞瞬时表达酪氨酸羟化酶（TH），第一种酶的儿茶酚胺生物合成途径，是目前在胰腺的小鼠胚胎从产前第11天（E11），并在E12，一些TH细胞含有胰高血糖素。在成人中也发现了含有TH的细胞，与胚胎的TH细胞不同，成人中不含有胰高血糖素（G。Teitelman，T. H. Joh和D. J. Reis（1981）.Proc. Natl. Acad. Sci.78，5225）。本研究应用免疫细胞化学和放射自显影技术，观察了胚胎胰腺TH细胞是否存在于胰腺的整个发育过程中，（B）是否定位于胰腺的特定区域，（c）是否随时含有胰岛素，（d）是否增殖。我们发现TH细胞终生存在于胰腺中。在胚胎中，含有TH的细胞仅沿着胰管定位，也含有胰高血糖素或胰岛素，并且能够增殖。相反，出生后，胰管不含TH细胞。出生后和成年小鼠中含有TH的细胞也不同于胚胎TH细胞，因为它们在所有胰岛中发现，含有胰岛素但不含胰高血糖素，并且不合成DNA，因此不增殖。这些发现表明，祖细胞含有儿茶酚胺，并存在于胰管引起胰高血糖素和胰岛素细胞的成年胰岛。他们还指出，出生后和成年小鼠的TH-胰岛素细胞不是干细胞，而是出生后出现在胰岛中的有丝分裂后细胞。
We have previously reported that cells transiently expressing tyrosine hydroxylase (TH), the first enzyme of the catecholamine biosynthetic pathway, are present in the pancreas of mouse embryos from prenatal Day 11 (E11) and that, at E12, some TH cells contain glucagon. Cells containing TH were also found in adults which, unlike the TH cells of embryos, did not contain glucagon (G. Teitelman, T. H. Joh, and D. J. Reis (1981).Proc. Natl. Acad. Sci.78,5225). These findings suggested to us that the TH cells of embryonic pancreas were the precursors of glucagon cells of adults.In this study we used immunocytochemical and autoradiographic techniques to determine whether cells containing TH (a) were present in pancreas throughout pre- and postnatal development, (b) were localized to a specific region of the gland, (c) contained insulin at any time, and (d) proliferated. We found that TH cells were present in pancreas throughout life. In embryos, cells containing TH localized only along the pancreatic duct, also contained either glucagon or insulin, and were able to proliferate. In contrast, after birth, the pancreatic duct contained no TH cells. Cells containing TH in postnatal and adult mice also differed from embryonic TH cells in that they were found in all islets, contained insulin but not glucagon, and did not synthesize DNA, and hence did not proliferate. These findings suggest that progenitor cells that contain catecholamines and are present in the pancreatic duct give rise to glucagon and insulin cells of adult islets. They also indicate that the TH-insulin cells of postnatal and adult mice are not stem cells but are postmitotic cells that appear in the islets after birth.
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大鼠脑中酪氨酸羟化酶——辅因子需求、区域和亚细胞分布。
影响因子：
5.8
作者：
J. Coyle
通讯作者：
J. Coyle
胆碱能鸡睫状神经节儿茶酚胺能特征的部分表达：体内和体外研究。
影响因子：
2.7
作者：
L. Iacovitti;T. Joh;V. Albert;D. H. Park;D. Reis;G. Teitelman
通讯作者：
G. Teitelman
[125I]-二级抗血清的放射自显影检测：一种与过氧化物酶免疫细胞化学兼容的敏感光和电子显微镜标记方法，用于神经元抗原的双重定位。
DOI：
10.1177/34.6.2422251
发表时间：
1986
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Pickel,VM;Chan,J;Milner,TA
通讯作者：
Milner,TA
儿茶酚胺能前体转化为小鼠胚胎胰腺中的胰高血糖素 (A) 细胞。
DOI：
10.1073/pnas.78.8.5225
发表时间：
1981
影响因子：
11.1
作者：
Teitelman,G;Joh,TH;Reis,DJ
通讯作者：
Reis,DJ
DOI：
--
发表时间：
1969
期刊：
影响因子：
--
作者：
U. Drews;E. Kussäther;K. Usadel *
通讯作者：
K. Usadel *