A Transcriptomic Analysis Reveals Novel Patterns of Gene Expression During 3T3-L1 Adipocyte Differentiation.
A Transcriptomic Analysis Reveals Novel Patterns of Gene Expression During 3T3-L1 Adipocyte Differentiation.
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转录组分析揭示 3T3-L1 脂肪细胞分化过程中基因表达的新模式
DOI：
10.3389/fmolb.2020.564339
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发表时间：
2020
影响因子：
5
通讯作者：
Zhu T
中科院分区：
文献类型：
--
作者：
Sun W;Yu Z;Yang S;Jiang C;Kou Y;Xiao L;Tang S;Zhu T
背景肥胖的特征是脂肪组织质量增加，这是由于脂肪细胞大小（肥大）和数量（增生）增加所致。控制脂肪细胞的调节和分化的分子机制对于更好地理解肥胖的病理机制起着至关重要的作用。然而，脂肪细胞分化的机制仍不清楚。目的从转录组水平比较脂肪细胞分化过程中基因表达的变化，为进一步了解脂肪细胞分化的机制提供依据。方法采用RNA测序技术、GO和KEGG分析、定量RT-PCR和油红O染色等方法。结果在3 T3-L1细胞的两个分化阶段中，有大量基因表达上调或下调。基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析表明，脂肪细胞分化的全过程主要涉及脂质代谢和氧化还原反应。差异表达谱分析表明，在脂肪细胞分化的晚期，免疫应答和细胞周期粘附下降。此外，定量RT-PCR结果显示，在分化的脂肪细胞中，Trpv 4、Trpm 4、Trpm 5和Trpm 7的mRNA表达水平显著降低。另一方面，在分化的脂肪细胞中，Trpv 1、Trpv 2、Trpv 6和Trpc 1的mRNA表达水平显著增加。此外，TRPV 2和TRPM 7的mRNA表达在饮食诱导的小鼠皮下白色脂肪组织中也显著增加。此外，TRPM 7、TRPV 1和TRPV 2的激活抑制脂肪细胞的分化。结论这些数据描述了脂肪细胞分化过程中转录谱的变化，为深入分析脂肪细胞分化的可能机制提供了依据。这些数据为脂肪细胞分化机制的理解提供了新的见解。
Background Obesity is characterized by increased adipose tissue mass that results from increased fat cell size (hypertrophy) and number (hyperplasia). The molecular mechanisms that govern the regulation and differentiation of adipocytes play a critical role for better understanding of the pathological mechanism of obesity. However, the mechanism of adipocyte differentiation is still unclear. Objective The present study aims to compare the gene expression changes during adipocyte differentiation in the transcriptomic level, which may help to better understand the mechanism of adipocyte differentiation. Methods RNA sequencing (RNA-seq) technology, GO and KEGG analysis, quantitative RT-PCR, and oil red O staining methods were used in this study. Results A lot of genes were up- or down-regulated between each two differentiation stages of 3T3-L1 cells. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that lipid metabolism and oxidation–reduction reaction were mainly involved in the whole process of adipocyte differentiation. Decreased immune response and cell cycle adhesion occurred in the late phase of adipocyte differentiation, which was demonstrated by divergent expression pattern analysis. Moreover, quantitative RT-PCR results showed that the mRNA expression levels of Trpv4, Trpm4, Trpm5, and Trpm7 were significantly decreased in the differentiated adipocytes. On the other hand, the mRNA expression levels of Trpv1, Trpv2, Trpv6, and Trpc1 were significantly increased in the differentiated adipocytes. Besides, the mRNA expressions of TRPV2 and TRPM7 were also significantly increased in subcutaneous white adipose tissue from diet-induced mice. In addition, the activation of TRPM7, TRPV1, and TRPV2 suppressed the differentiation of adipocytes. Conclusion These data present the description of transcription profile changes during adipocyte differentiation and provides an in-depth analysis of the possible mechanisms of adipocyte differentiation. These data offer new insight into the understanding of the mechanisms of adipocyte differentiation.
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DOI：
10.1016/j.bbrc.2008.06.077
发表时间：
2008-09-05
影响因子：
3.1
作者：
Ahn, Jiyun;Lee, Hyunjung;Ha, Taleyoul
通讯作者：
Ha, Taleyoul
TRP通道基因在小鼠中的组织特异性表达及其在三种不同小鼠菌株中的变异。
影响因子：
4.4
作者：
Kunert-Keil, Christiane;Bisping, Frederike;Kruger, Jana;Brinkmeier, Heinrich
通讯作者：
Brinkmeier, Heinrich
影响因子：
4.6
作者：
Liu Y;Ni BJ
通讯作者：
Ni BJ
DOI：
10.1152/ajpregu.00368.2004
发表时间：
2004-12-01
影响因子：
2.8
作者：
Cammisotto, PG;Bukowiecki, LJ
通讯作者：
Bukowiecki, LJ
影响因子：
16.2
作者：
MONTELL, C;RUBIN, GM
通讯作者：
RUBIN, GM