Engineering New Aptamer Geometries for Electrochemical Aptamer-Based Sensors.
Engineering New Aptamer Geometries for Electrochemical Aptamer-Based Sensors.
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DOI：
10.1117/12.820419
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Plaxco KW
中科院分区：
文献类型：
--
作者：
White RJ;Plaxco KW
基于电化学适配子的传感器(E-AB传感器)代表了一种很有前途的小分子检测新方法。E-AB传感器包括一端连接到电极表面的适配子。用电活性氧化还原标记物修饰被探测适配子的远端以进行信号转导。在这里，我们报道了通过优化适体的几何结构来优化可卡因检测E-AB传感器。我们探索了两种新的适配子结构，一种是将三个可卡因适配子串联成一个多适配子，另一种是将可卡因适配子分成几个片段，通过一个非结构化的60-胸腺嘧啶接头连接。这两种结构的设计使得报告氧化还原标签在展开的、无靶标的构象中将位于离电极更远的位置。与此一致，我们发现这两种结构的信号增益比原始E-AB结构的信号增益高两到三倍。同样，所有三种体系结构都具有足够的选择性，可以直接部署在复杂的样本矩阵中，例如未稀释的全血，所有三种传感器都成功地检测到可卡因的存在。这项正在进行的研究中的发现应该对未来优化基于电化学适配子的传感器的信号传递有价值。
Electrochemical aptamer-based sensors (E-AB sensors) represent a promising new approach to the detection of small molecules. E-AB sensors comprise an aptamer that is attached at one end to an electrode surface. The distal end of the aptamer probed is modified with an electroactive redox marker for signal transduction. Herein we report on the optimization of a cocaine-detecting E-AB sensor via optimization of the geometry of the aptamer. We explore two new aptamer architectures, one in which we concatenate three cocaine aptamers into a poly-aptamer and a second in which we divide the cocaine aptamer into pieces connected via an unstructured, 60-thymine linker. Both of these structures are designed such that the reporting redox tag will be located farther from the electrode in the unfolded, target-free conformation. Consistent with this, we find that signal gains of these two constructs are two to three times higher than that of the original E-AB architecture. Likewise all three architectures are selective enough to deploy directly in complex sample matrices, such as undiluted whole blood, with all three sensors successfully detecting the presence of cocaine. The findings in this ongoing study should be of value in future efforts to optimize the signaling of electrochemical aptamer-based sensors.
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影响因子：
14.8
作者：
Xiao, Yi;Lai, Rebecca Y.;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
DOI：
10.1021/la800801v
发表时间：
2008-09-16
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
White RJ;Phares N;Lubin AA;Xiao Y;Plaxco KW
通讯作者：
Plaxco KW
影响因子：
8.4
作者：
Bozic, Robert G.;West, Alan C.;Levicky, Rastislav
通讯作者：
Levicky, Rastislav
影响因子：
15
作者：
Stojanovic, MN;de Prada, P;Landry, DW
通讯作者：
Landry, DW
影响因子：
15
作者：
Xiao, Yi;Rowe, Aaron A.;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.