Mast cells are critical for controlling the bacterial burden and the healing of infected wounds
Mast cells are critical for controlling the bacterial burden and the healing of infected wounds
复制标题
肥大细胞对于控制细菌负荷和感染伤口的愈合至关重要
DOI：
10.1073/pnas.1908816116
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Siebenhaar F
中科院分区：
文献类型：
--
作者：
Zimmermann C;Troeltzsch D;Giménez-Rivera VA;Galli SJ;Metz M;Maurer M;Siebenhaar F
皮肤伤口感染是一个严重的健康问题，抗生素耐药性正在上升。肥大细胞（MC）已被证明有助于在某些细菌感染的宿主防御反应，但其在皮肤伤口双重感染的作用是未知的。我们将2个MC缺陷小鼠品系进行铜绿假单胞菌伤口感染，发现感染皮肤伤口的伤口闭合明显延迟。这种延迟与在没有MC的情况下细菌清除受损有关。MC的植入恢复了细菌清除和伤口闭合。细菌杀伤依赖于从MC释放的IL-6，并且用IL-6缺陷的MC植入未能控制伤口感染。用重组IL-6治疗增强了细菌杀伤，并导致体内伤口感染和正常伤口愈合的控制。综上所述，我们的研究结果证明了增强宿主先天免疫应答的防御机制，即MC衍生的IL-6对角质形成细胞抗菌功能的影响。
Skin wound infections are a significant health problem, and antibiotic resistance is on the rise. Mast cells (MCs) have been shown to contribute to host–defense responses in certain bacterial infections, but their role in skin wound superinfection is unknown. We subjected 2 MC-deficient mouse strains toPseudomonas aeruginosaskin wound infection and found significantly delayed wound closure in infected skin wounds. This delay was associated with impaired bacterial clearance in the absence of MCs. Engraftment of MCs restored both bacterial clearance and wound closure. Bacterial killing was dependent on IL-6 released from MCs, and engraftment with IL-6–deficient MCs failed to control wound infection. Treatment with recombinant IL-6 enhanced bacterial killing and resulted in the control of wound infection and normal wound healing in vivo. Taken together, our results demonstrate a defense mechanism for boosting host innate immune responses, namely effects of MC-derived IL-6 on antimicrobial functions of keratinocytes.
影响因子：
6.5
作者：
Gallo, RL;Dorschner, RA;Bernfield, M
通讯作者：
Bernfield, M
影响因子：
3.6
作者：
Choi HW;Abraham SN
通讯作者：
Abraham SN