ACOT7 protects epidermal stem cells against lipid peroxidation.
ACOT7 protects epidermal stem cells against lipid peroxidation.
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ACOT7 保护表皮干细胞免受脂质过氧化。
DOI：
10.1007/s11626-022-00703-9
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发表时间：
2022-08
影响因子：
2.1
通讯作者：
Yin, Siyuan
中科院分区：
文献类型：
--
作者：
Zhang, Guang;Ma, Jiaxu;Wu, Zhenjie;Cao, Guoqi;Liu, Chunyan;Song, Ru;Sun, Rui;Chen, Aoyu;Wang, Yibing;Yin, Siyuan
表皮干细胞（ESCs）对皮肤再生和修复至关重要。先前的研究表明，ESCs易受氧化应激的影响，从而导致脂质过氧化，影响皮肤修复。我们的研究旨在探讨ESCs如何抵抗脂质过氧化。通过蛋白质组学分析，我们发现酰基辅酶a硫酯酶7 （ACOT7）的表达与转铁蛋白浓度呈正相关。构建ACOT7过表达腺病毒载体，转染ESCs。流式细胞术测定脂质过氧化水平、细胞活力和丙二醛水平。结果显示，ACOT7能抑制脂质过氧化，降低丙二醛（MDA）水平，提高H2O2、Erastin和RSL3诱导的内皮细胞存活率。我们的数据表明ACOT7具有保护ESCs免受铁依赖性脂质过氧化的作用。
Epidermal stem cells (ESCs) are critical for skin regeneration and repair. Previous studies have shown that ESCs are susceptible to oxidative stress, which in turn leads to lipid peroxidation and affects skin repair. Our study aims to explore how ESCs resist lipid peroxidation. By performing proteomics analysis, we found that the expression of Acyl-CoA thioesterase 7 (ACOT7) was positively correlated with the concentration of transferrin. Overexpression adenovirus vectors of ACOT7 were constructed and transfected into ESCs. Levels of lipid peroxidation by flow cytometry, cell viabilities, and MDA levels were measured. The results revealed that ACOT7 could inhibit lipid peroxidation, reduce the level of malondialdehyde (MDA), and improve the survival rate of ESCs induced by H2O2, Erastin, and RSL3. Our data suggest that ACOT7 has an effect on protecting ESCs against iron-dependent lipid peroxidation.
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影响因子：
14.8
作者：
Doll S;Proneth B;Tyurina YY;Panzilius E;Kobayashi S;Ingold I;Irmler M;Beckers J;Aichler M;Walch A;Prokisch H;Trümbach D;Mao G;Qu F;Bayir H;Füllekrug J;Scheel CH;Wurst W;Schick JA;Kagan VE;Angeli JP;Conrad M
通讯作者：
Conrad M
影响因子：
14.8
作者：
Kagan VE;Mao G;Qu F;Angeli JP;Doll S;Croix CS;Dar HH;Liu B;Tyurin VA;Ritov VB;Kapralov AA;Amoscato AA;Jiang J;Anthonymuthu T;Mohammadyani D;Yang Q;Proneth B;Klein-Seetharaman J;Watkins S;Bahar I;Greenberger J;Mallampalli RK;Stockwell BR;Tyurina YY;Conrad M;Bayır H
通讯作者：
Bayır H
影响因子：
64.5
作者：
Dixon SJ;Lemberg KM;Lamprecht MR;Skouta R;Zaitsev EM;Gleason CE;Patel DN;Bauer AJ;Cantley AM;Yang WS;Morrison B 3rd;Stockwell BR
通讯作者：
Stockwell BR
影响因子：
5.6
作者：
Wright JA;Richards T;Srai SK
通讯作者：
Srai SK
影响因子：
5.6
作者：
Sui, Xinbing;Zhang, Ruonan;Xie, Tian
通讯作者：
Xie, Tian