Identifying Isl1 Genetic Lineage in the Developing Olfactory System and in GnRH-1 Neurons.
Identifying Isl1 Genetic Lineage in the Developing Olfactory System and in GnRH-1 Neurons.
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在发育中的嗅觉系统和GNRH-1神经元中鉴定ISL1遗传谱系。
DOI：
10.3389/fphys.2020.601923
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发表时间：
2020
影响因子：
4
通讯作者：
Forni PE
中科院分区：
文献类型：
--
作者：
Taroc EZM;Katreddi RR;Forni PE
在胚胎发育过程中，对称的外胚层增厚[嗅板（OP）]产生了几种组成嗅觉系统的细胞类型，例如形成末端神经节（TN）、促性腺激素释放激素-1神经元（GnRH-1 ns）和啮齿动物中其他迁移神经元的细胞类型。尽管记录了这些细胞类型之间的遗传异质性，但仍存在由OP产生的未鉴定的细胞群。一个候选人，以确定基板衍生的神经元在发展中的鼻区是转录因子Isl 1，这是最近确定的GnRH-3神经元的终末神经在鱼，以及表达在神经元的鼻迁移质量（MM）。在这里，我们分析了Isl 1遗传谱系的化学感觉神经元群体在鼻区和迁移GnRH-1 ns在小鼠中使用原位杂交，免疫标记的他莫昔芬诱导Isl 1CreERT和组成性Isl 1Cre敲入小鼠品系。此外，我们还在发育中的GnRH神经元中进行了条件性Isl 1消融。我们发现Isl 1谱系跨越呼吸上皮的非感觉细胞和OE和VNO的支持细胞。我们确定了一个人口的短暂胚胎Isl 1+神经元的嗅上皮和稀疏Isl 1+神经元出生后的VNO。Isl 1在几乎所有的GnRH神经元和大约一半的其他神经元群体的MM中表达。然而，Isl 1条件性消融单独不显着损害GnRH-1神经元迁移或GnRH-1表达，提示代偿机制。进一步的研究将阐明Isl 1在迁移性内分泌神经元发育中的功能和机制作用。
During embryonic development, symmetric ectodermal thickenings [olfactory placodes (OP)] give rise to several cell types that comprise the olfactory system, such as those that form the terminal nerve ganglion (TN), gonadotropin releasing hormone-1 neurons (GnRH-1ns), and other migratory neurons in rodents. Even though the genetic heterogeneity among these cell types is documented, unidentified cell populations arising from the OP remain. One candidate to identify placodal derived neurons in the developing nasal area is the transcription factor Isl1, which was recently identified in GnRH-3 neurons of the terminal nerve in fish, as well as expression in neurons of the nasal migratory mass (MM). Here, we analyzed the Isl1 genetic lineage in chemosensory neuronal populations in the nasal area and migratory GnRH-1ns in mice using in situ hybridization, immunolabeling a Tamoxifen inducible Isl1CreERT and a constitutive Isl1Cre knock-in mouse lines. In addition, we also performed conditional Isl1 ablation in developing GnRH neurons. We found Isl1 lineage across non-sensory cells of the respiratory epithelium and sustentacular cells of OE and VNO. We identified a population of transient embryonic Isl1 + neurons in the olfactory epithelium and sparse Isl1 + neurons in postnatal VNO. Isl1 is expressed in almost all GnRH neurons and in approximately half of the other neuron populations in the MM. However, Isl1 conditional ablation alone does not significantly compromise GnRH-1 neuronal migration or GnRH-1 expression, suggesting compensatory mechanisms. Further studies will elucidate the functional and mechanistic role of Isl1 in development of migratory endocrine neurons.
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影响因子：
64.5
作者：
Greer PL;Bear DM;Lassance JM;Bloom ML;Tsukahara T;Pashkovski SL;Masuda FK;Nowlan AC;Kirchner R;Hoekstra HE;Datta SR
通讯作者：
Datta SR
影响因子：
13.6
作者：
通讯作者：
--
影响因子：
4.1
作者：
Balasubramanian, Ravikumar;Dwyer, Andrew;Crowley, William F., Jr.
通讯作者：
Crowley, William F., Jr.
影响因子：
30.8
作者：
Dodé, C;Levilliers, J;Hardelin, JP
通讯作者：
Hardelin, JP
影响因子：
3.3
作者：
Fornaro, M;Geuna, S;Giacobini-Robecchi, MG
通讯作者：
Giacobini-Robecchi, MG