The synergistic effect of organic silicone quaternary ammonium salt and 5-fluorouracil on hepatocellular carcinoma in vitro and in vivo
The synergistic effect of organic silicone quaternary ammonium salt and 5-fluorouracil on hepatocellular carcinoma in vitro and in vivo
复制标题
有机硅季铵盐与5-氟尿嘧啶对肝细胞癌体内外协同作用
DOI：
10.1097/cej.0b013e328364f2c8
复制
发表时间：
2014-09
影响因子：
2.4
通讯作者：
Wang Zhi-Ping
中科院分区：
文献类型：
--
作者：
Zhang Jun;Ding Hui;Wang Dong-Min;Wang Zhi-Ping
肝细胞癌（HCC）是全球第三大常见癌症，每年造成超过37万人死亡，其中约一半在中国。化疗是晚期HCC患者的最佳治疗方法，尽管化疗耐药性已成为成功进行肝癌手术的重要障碍。本文通过评价药物特性，探讨有机硅季铵盐（洁优神）与5-氟尿嘧啶（5-FU）单独及联合作用的效果。MTT法结果表明，结疣参与5-FU单独及联合作用均能抑制肝癌细胞株的增殖，并呈剂量依赖性和时间依赖性。电镜和Hoechst 33342染色显示，在凋亡细胞中的特征性凋亡小体与结优参和5-FU处理。流式细胞仪检测结果显示，G 0/G1期细胞比例逐渐增加，S期细胞比例逐渐减少。综上所述，这些结果表明，联合应用结友参与5-FU对HCC生长具有协同抗癌作用，靶向治疗策略可能会提高HCC对化疗的敏感性。
Hepatocellular carcinoma (HCC) is the third most common type of cancer worldwide, causing over 370 000 deaths per year, with approximately half of them in China. Chemotherapy is the optimal treatment for patients with advanced HCC, although chemoresistance has become a significant obstacle to successful liver cancer surgery. In this paper, we have assessed the characteristics of drugs to explore the effects of individual and combined action of organic silicone quaternary ammonium salt (Jieyoushen) and 5-fluorouracil (5-FU). The results of MTT assays showed that single and combined action of Jieyoushen and 5-FU can inhibit the proliferation of liver carcinoma cell lines in a dose-dependent and time-dependent manner, respectively. Electron microscopy and Hoechst 33342 staining showed characteristic apoptotic bodies in apoptotic cells treated with Jieyoushen and 5-FU. Flow cytometry results indicated that the percentage of cells at G0/G1 phase gradually increased, whereas it gradually decreased during the S phase after treatment. Taken together, these results suggest that the combination of Jieyoushen with 5-FU exerts a synergistic anticancer effect on HCC growth and that targeted therapeutic strategies may improve HCC sensitivity to chemotherapy.
登录
查看更多内容
影响因子：
2
作者：
H. Cao;H. Phan;Li-xi Yang
通讯作者：
H. Cao;H. Phan;Li-xi Yang
影响因子：
5.8
作者：
Ramalingam, Suresh;Belani, Chandra
通讯作者：
Belani, Chandra
影响因子：
5.8
作者：
Jia, Huijie;Li, Yang;Xu, De Qi
通讯作者：
Xu, De Qi
Opsin3通过调节细胞凋亡途径使肝细胞癌细胞对5-氟尿嘧啶治疗敏感
影响因子：
9.7
作者：
Jiao, Jianhua;Hong, Shanchao;Zhu, Changliang
通讯作者：
Zhu, Changliang
DOI：
--
发表时间：
2010
期刊：
Journal of Chongqing Medical University
影响因子：
--
作者：
L. Chuan
通讯作者：
L. Chuan