Potentiation of adenosine triphospate‐induced contractile responses of the guinea‐pig isolated vas deferens by adenosine monophosphate and adenosine 5′‐monophosphorothioate
Potentiation of adenosine triphospate‐induced contractile responses of the guinea‐pig isolated vas deferens by adenosine monophosphate and adenosine 5′‐monophosphorothioate
复制标题
单磷酸腺苷和 5-单硫代磷酸腺苷增强豚鼠离体输精管的三磷酸腺苷诱导的收缩反应
DOI：
10.1111/j.1476-5381.1987.tb11257.x
复制
发表时间：
1987
影响因子：
7.3
通讯作者：
J. Fedan
中科院分区：
文献类型：
--
作者：
J. Fedan
1比较腺苷核苷酸(三磷酸腺苷、二磷酸腺苷和一磷酸腺苷)或其硫代类似物(腺苷5‘-0-(3-硫代三磷酸)，γS；腺苷5’-0-(2-硫代二磷酸盐)，腺苷βS；腺苷5‘-一磷酸腺苷(AMPαS))对三磷酸腺苷收缩反应的影响。2用低(1μm)或高(30 0μm)三磷酸腺苷刺激获得对照反应后，将一对输精管与其中一个腺嘌呤核苷酸孵育5min，对侧输精管与相应的硫代磷酸类似物孵育。在孵育结束时，用三磷酸腺苷再次挑战这些制剂。3AMP或AMPα与S孵育后，对1μm和30 0μm三磷酸腺苷的反应出现一过性增强。与AMPαS孵育后的增强作用大于AMP的作用。4二磷酸腺苷、二磷酸腺苷βS、三磷酸腺苷和三磷酸腺苷γS孵育后，对1μm三磷酸腺苷的反应减弱，而对30 0μm三磷酸腺苷的反应无影响。5因此，与腺苷三磷酸和腺苷αS孵育后，腺苷三磷酸腺苷诱导的反应得到增强，而不是抑制。另一方面，5‘-二磷酸、5’-三磷酸、5‘-O-(2-硫代二磷酸)和5’-O-(3-硫代三磷酸)对腺苷没有影响或引起自身抑制。6以上结果表明，AMP对外源ATP的反应性有增强作用。ATP的酶降解产生的AMP可能调节内源释放的ATP作为共递质的反应水平。
1 The effects of incubating the guinea‐pig isolated vas deferens in the presence of adenine nucleotides (adenosine triphosphate, ATP; adenosine diphosphate, ADP; and adenosine monophosphate, AMP), or in the presence of their phosphorothioate analogues (adenosine 5′‐0‐(3‐thiotriphosphate), ATPγS; adenosine 5′‐0‐(2‐thiodipnosphate), ADPβS; and adenosine 5′‐monophosphorothioate, AMPαS), on contractile responses to ATP were compared. 2 After challenge with a low (1 μm) or high (300 μm) concentration of ATP to obtain control responses, one vas deferens of a pair was incubated for 5 min with one of the adenine nucleotides, while the contralateral preparation was incubated with the corresponding phosphorothioate analogue. At the conclusion of the incubation the preparations were challenged again with ATP. 3 Incubation with AMP or AMPαS resulted in a transient potentiation of responses to 1 μm and 300 μm ATP. The potentiation following incubation with AMPαS was larger than that produced by AMP. 4 After incubation with ADP, ADPβS, ATP and ATPγS, responses to 1 μm ATP were decreased, while those to 300 μm ATP were unaffected. 5 Thus, incubation with AMP and AMPαS results in potentiation, rather than inhibition, of ATP‐induced responses. On the other hand, 5′‐diphosphate, 5′‐triphosphate, 5′‐O‐(2‐thiodiphosphate) and 5′‐O‐(3‐thiotriphosphate) moieties on adenosine have no effect or cause autoinhibition. 6 These results indicate that AMP exerts a potentiating effect on reactivity to exogenous ATP. AMP arising from the enzymatic degradation of ATP might modulate the level of response to ATP released endogenously as a cotransmitter.
影响因子：
5
作者：
FEDAN, JS;HOGABOOM, GK;WESTFALL, DP
通讯作者：
WESTFALL, DP
ATP及相关核苷酸诱导豚鼠输精管平滑肌收缩的比较。
影响因子：
5
作者：
Fedan,JS;Hogaboom,GK;Westfall,DP;O'Donnell,JP
通讯作者：
O'Donnell,JP