Purification and characterization of a 59-kilodalton protein that specifically binds to NF-kappa B-binding motifs of the defense protein genes of Sarcophaga peregrina (the flesh fly)
Purification and characterization of a 59-kilodalton protein that specifically binds to NF-kappa B-binding motifs of the defense protein genes of Sarcophaga peregrina (the flesh fly)
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59 千道尔顿蛋白质的纯化和表征，该蛋白质与 Sarcophaga peregrina（肉蝇）防御蛋白基因的 NF-kappa B 结合基序特异性结合
DOI：
10.1128/mcb.13.7.4049-4056.1993
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发表时间：
1993
影响因子：
5.3
通讯作者：
Shunji Natori
中科院分区：
文献类型：
--
作者：
Ayako Kobayashi;Mayumi Matsui;T. Kubo;Shunji Natori
当NIH-Sape-4细胞与细菌脂多糖或β-1，3-葡聚糖一起培养时，各种Sarcophaga peregrina（肉蝇）防御蛋白基因被激活。发现防御蛋白基因的5'上游区域具有显示与哺乳动物NF-κ B结合共有序列相似的共同基序。鉴定了一种对嗜肉蝇属凝集素启动子的NF-κ B结合基序具有亲和力的蛋白质，并纯化至接近同质性。这种59-kDa蛋白质还结合到其他防御蛋白基因的NF-κ B结合基序，例如，sarcotoxin I和sarcotoxin II基因。这种蛋白质被发现在细胞的细胞质和细胞核的部分，它似乎从细胞质迁移到细胞核处理的细胞与脂多糖。该59-kDa蛋白可能是S.佩雷格里娜
Various Sarcophaga peregrina (flesh fly) defense protein genes were shown to be activated when NIH-Sape-4 cells were cultured with bacterial lipopolysaccharides or beta-1,3-glucan. The 5' upstream regions of the defense protein genes were found to have common motifs showing similarity to the mammalian NF-kappa B-binding consensus sequence. A protein with affinity to the NF-kappa B-binding motif of the Sarcophaga lectin promoter was identified and purified to near homogeneity. This 59-kDa protein also bound to the NF-kappa B-binding motifs of other defense protein genes, e.g., sarcotoxin I and sarcotoxin II genes. This protein was found in both the cytoplasmic and the nuclear fractions of the cells, and it appeared to migrate from the cytoplasm to the nucleus on treatment of the cells with lipopolysaccharides. This 59-kDa protein is probably a transcriptional regulator of the genes for defense proteins of S. peregrina.
鉴定和纯化人免疫球蛋白增强子结合蛋白 (NF-kappa B)，该蛋白可在体外激活人类免疫缺陷病毒 1 型启动子的转录。
DOI：
10.1073/pnas.85.13.4700
发表时间：
1988
影响因子：
11.1
作者：
Kawakami,K;Scheidereit,C;Roeder,RG
通讯作者：
Roeder,RG