Biocatalytic Approaches to Building Blocks for Enzymatic and Chemical Glycan Synthesis.
Biocatalytic Approaches to Building Blocks for Enzymatic and Chemical Glycan Synthesis.
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DOI：
10.1021/jacsau.2c00529
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发表时间：
2023-01-23
期刊：
影响因子：
8
通讯作者：
Miller, Gavin J
中科院分区：
文献类型：
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作者：
Dolan, Jonathan P;Cosgrove, Sebastian C;Miller, Gavin J
虽然生物催化领域在过去的20-30年里蓬勃发展，但对碳水化合物活性酶，特别是参与聚糖结构单元生物合成的糖核苷酸的理解和改进的进展相对较慢。这种观点强调需要进一步深入了解底物混杂和使用生物催化的基本原理（合理的设计，定向进化，固定化），以扩大底物范围对这样的碳水化合物积木合成和/或提高酶的稳定性，动力学，或营业额。此外，它还探讨了使用生物催化提供简单，具有成本效益的立体化学定义的碳水化合物材料，可以进行后期化学功能化或自动化聚糖合成/聚合的增长前提。
While the field of biocatalysis has bloomed over the past 20–30 years, advances in the understanding and improvement of carbohydrate-active enzymes, in particular, the sugar nucleotides involved in glycan building block biosynthesis, have progressed relatively more slowly. This perspective highlights the need for further insight into substrate promiscuity and the use of biocatalysis fundamentals (rational design, directed evolution, immobilization) to expand substrate scopes toward such carbohydrate building block syntheses and/or to improve enzyme stability, kinetics, or turnover. Further, it explores the growing premise of using biocatalysis to provide simple, cost-effective access to stereochemically defined carbohydrate materials, which can undergo late-stage chemical functionalization or automated glycan synthesis/polymerization.
DOI：
10.1002/anie.202008067
发表时间：
2020-12-07
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
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作者：
Marchesi A;Parmeggiani F;Louçano J;Mattey AP;Huang K;Gupta T;Salwiczek M;Flitsch SL
通讯作者：
Flitsch SL