A Pseudo-Atomic Model of the COPII Cage Obtained from CryoEM and Mass Spectrometry Analyses
A Pseudo-Atomic Model of the COPII Cage Obtained from CryoEM and Mass Spectrometry Analyses
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通过 CryoEM 和质谱分析获得的 COPII 笼的伪原子模型
DOI：
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
S. Stagg
中科院分区：
文献类型：
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作者：
A. Noble;Qian Zhang;J. O'Donnell;H. Hariri;N. Bhattacharya;A. Marshall;S. Stagg
COPII vesicles transport proteins from the endoplasmic reticulum to the Golgi apparatus. Previous COPII-cage cryo-EM structures lacked the resolution necessary to determine the residues of Sec13 and Sec31 that mediate assembly and flexibility of the COPII cage. Here we present a 12-Å structure of the human COPII cage, where the tertiary structure of Sec13 and Sec31 is clearly identifiable. We employ this structure and a homology model of the Sec13–Sec31 complex to create a reliable pseudoatomic model of the COPII cage. We combined this model with hydrogen/deuterium-exchange MS analysis to characterize four distinct contact regions at the vertices of the COPII cage. Furthermore, we found that the two-fold symmetry of the Sec31 dimeric region in Sec13–Sec31 is broken upon cage formation and that the resulting hinge is essential to form the proper edge geometry in COPII cages.
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影响因子：
7.4
作者：
Zhang, Qian;Willison, LeAnna N.;Tripathi, Pallavi;Sathe, Shridhar K.;Roux, Kenneth H.;Emmett, Mark R.;Blakney, Greg T.;Zhang, Hui-Min;Marshall, Alan G.
通讯作者：
Marshall, Alan G.
影响因子：
3
作者：
Suloway, C;Pulokas, J;Carragher, B
通讯作者：
Carragher, B
影响因子：
3
作者：
Lander, Gabriel C.;Stagg, Scott M.;Voss, Neil R.;Cheng, Anchi;Fellmann, Denis;Pulokas, James;Yoshioka, Craig;Irving, Christopher;Mulder, Anke;Lau, Pick-Wei;Lyumkis, Dmitry;Potter, Clinton S.;Carragher, Bridget
通讯作者：
Carragher, Bridget
影响因子：
5.7
作者：
Trabuco, Leonardo G.;Villa, Elizabeth;Schulten, Klaus
通讯作者：
Schulten, Klaus
影响因子：
3
作者：
Frank, J;Radermacher, M;Leith, A
通讯作者：
Leith, A