Comparison of faecal microbiota in Blastocystis-positive and Blastocystis-negative irritable bowel syndrome patients.
Comparison of faecal microbiota in Blastocystis-positive and Blastocystis-negative irritable bowel syndrome patients.
复制标题
DOI：
10.1186/s40168-016-0191-0
复制
发表时间：
2016-08-31
期刊：
影响因子：
15.5
通讯作者：
Bielefeldt-Ohmann H
中科院分区：
文献类型：
--
作者：
Nagel R;Traub RJ;Allcock RJ;Kwan MM;Bielefeldt-Ohmann H
我们调查了IBS患者中囊胚的携带是否与粪便微生物群的差异有关。40例大肠杆菌为主的IBS患者（26例囊胚阳性和14例囊胚阴性）和57例健康对照（HC）（42例囊胚阳性和15例囊胚阴性）提交粪便样本进行16 S核糖体RNA基因的荟萃分析。从门到属的水平评价了这些IBS和HC组中细菌相对丰度的差异。在IBS患者中观察到两个优势门的显著变化，无论芽囊菌感染状态如何，即厚壁菌门的增加和拟杆菌门的相对丰度的统计学显著减少（厚壁菌门与拟杆菌门的比率增加三倍）。与HC相比，在IBS受试者中还观察到许多细菌物种在属水平上的显著差异。然而，对囊胚阳性和囊胚阴性受试者的进一步临床亚组分析（无论症状如何）显示，IBS-P与IBS-N相比在门或属水平上无显著差异。肠道菌群占主导地位的IBS患者和健康对照之间的粪便微生物群的显着差异得到证实，但囊胚的携带并没有显着改变粪便微生物群。如果囊胚阳性患者代表IBS的一个单独的临床亚型，则该组不能通过微生物群的变化来识别。本文的在线版本（doi：10.1186/s40168-016-0191-0）包含补充材料，可供授权用户使用。
We investigated whether the carriage of Blastocystis in IBS patients was associated with differences in the faecal microbiota. Forty patients with diarrhoea-predominant IBS (26 Blastocystis-positive and 14 Blastocystis-negative) and 57 healthy controls (HC) (42 Blastocystis-positive and 15 Blastocystis-negative) submitted faecal samples for metataxonomic analysis of the 16S ribosomal RNA gene. Differences in the relative abundance of bacteria in these IBS and HC groups were evaluated from phylum to genus level. Significant changes were observed in two dominant phyla in IBS patients, regardless of Blastocystis infection status, namely a rise in Firmicutes and a statistically significant reduction in relative abundance of Bacteroidetes (with a threefold increase in the Firmicutes to Bacteoridetes ratio). Significant differences at genus level in IBS subjects compared to HC were also observed for many bacterial species. However, further clinical subgroup analysis of Blastocystis-positive and Blastocystis-negative subjects, regardless of symptoms, showed no significant differences at the phylum or genus level in IBS-P compared to IBS-N. Significant differences in the faecal microbiota between diarrhoea-predominant IBS patients and healthy controls were confirmed, but the carriage of Blastocystis did not significantly alter the faecal microbiota. If Blastocystis-positive patients represent a separate clinical subtype of IBS, this group is not identified by changes in the microbiota. The online version of this article (doi:10.1186/s40168-016-0191-0) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
7.3
作者：
Arrieta MC;Stiemsma LT;Amenyogbe N;Brown EM;Finlay B
通讯作者：
Finlay B
影响因子：
11
作者：
de Carcer, Daniel Aguirre;Cuiv, Paraic O.;Morrison, Mark
通讯作者：
Morrison, Mark
影响因子：
3.9
作者：
Canavan C;West J;Card T
通讯作者：
Card T
影响因子：
4.3
作者：
Lyra, Anna;Rinttila, Teemu;Palva, Airi
通讯作者：
Palva, Airi
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer