Cellulolytic potential of thermophilic species from four fungal orders.
Cellulolytic potential of thermophilic species from four fungal orders.
复制标题
DOI：
10.1186/2191-0855-3-47
复制
发表时间：
2013-08-19
期刊：
影响因子：
3.7
通讯作者：
Lange L
中科院分区：
文献类型：
--
作者：
Busk PK;Lange L
阐明真菌生物质降解是重要的了解周转的生物材料在自然界中，并具有重要意义的工业生物质转化。近年来，人们对阐明嗜热真菌的生物学作用及其工业上有用的酶的特性越来越感兴趣。在本研究中，我们调查了16个嗜热真菌的纤维素分解潜力，从三个子囊菌目Sordariales，Eurotiales和Onygenales和从放线菌目毛霉目，从而涵盖了所有的真菌订单，包括嗜热菌。嗜热真菌是唯一描述的可以在45°C以上的温度下生长的真核生物。所有16种真菌都能在结晶纤维素上生长，但它们分泌的酶表现出广泛不同的纤维素分解活性，最适pH和热稳定性。有趣的是，与以前的报道相反，我们发现一些真菌，如白黑果霉很容易生长在结晶纤维素上并产生纤维素酶。这些结果表明，有很大的差异，在同一物种的不同菌株的纤维素分解潜力。此外，所有选定的物种都能够降解纤维素，但在纤维素分解潜力和热稳定性的分泌组的差异没有相关的分类位置。对22个纤维素酶基因的PCR扩增和测序结果表明，不能从纤维素酶的系统发育关系推断其纤维素降解活性的热稳定性水平。
Elucidation of fungal biomass degradation is important for understanding the turnover of biological materials in nature and has important implications for industrial biomass conversion. In recent years there has been an increasing interest in elucidating the biological role of thermophilic fungi and in characterization of their industrially useful enzymes. In the present study we investigated the cellulolytic potential of 16 thermophilic fungi from the three ascomycete orders Sordariales, Eurotiales and Onygenales and from the zygomycete order Mucorales thus covering all fungal orders that include thermophiles. Thermophilic fungi are the only described eukaryotes that can grow at temperatures above 45°C. All 16 fungi were able to grow on crystalline cellulose but their secreted enzymes showed widely different cellulolytic activities, pH optima and thermostabilities. Interestingly, in contrast to previous reports, we found that some fungi such as Melanocarpus albomyces readily grew on crystalline cellulose and produced cellulases. These results indicate that there are large differences in the cellulolytic potential of different isolates of the same species. Furthermore, all the selected species were able to degrade cellulose but the differences in cellulolytic potential and thermostability of the secretome did not correlate to the taxonomic position. PCR amplification and sequencing of 22 cellulase genes from the fungi showed that the level of thermostability of the cellulose-degrading activity could not be inferred from the phylogenetic relationship of the cellulases.
登录
查看更多内容
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
3.8
作者：
HERR, D
通讯作者：
HERR, D
影响因子：
5
作者：
Hong, J;Tamaki, H;Kumagai, H
通讯作者：
Kumagai, H
影响因子：
1.5
作者：
Markowska-Szczupak, Agata;Ulfig, Krzysztof;Janda, Katarzyna
通讯作者：
Janda, Katarzyna
DOI：
10.3923/jm.2011.41.53
发表时间：
2011-01-01
期刊：
Research Journal of Microbiology
影响因子：
--
作者：
Karmakar, M.;Ray, R. R.
通讯作者：
Ray, R. R.