Theranostic cells: emerging clinical applications of synthetic biology.
Theranostic cells: emerging clinical applications of synthetic biology.
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DOI：
10.1038/s41576-021-00383-3
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发表时间：
2021-11
期刊：
影响因子：
--
通讯作者：
Silver PA
中科院分区：
文献类型：
--
作者：
McNerney MP;Doiron KE;Ng TL;Chang TZ;Silver PA
合成生物学试图重新设计生物系统，以便以可预测的方式执行新的功能。细菌和哺乳动物细胞工程的最新进展包括开发在生物样本中或在体内作为微创诊断或治疗的细胞，用于实时调节复杂的疾病状态。体外和体内基于细胞的生物传感器和治疗方法已经被开发出来，以针对包括癌症、微生物组失调以及自身免疫和代谢性疾病在内的广泛疾病。虽然益生菌疗法已经进入临床试验，但嵌合抗原受体(CAR)T细胞疗法已经获得监管部门的批准，证明了细胞疗法的临床潜力。该综述讨论了细菌和哺乳动物传感和药物输送平台的临床前和临床应用，以及可能实现新类别细胞诊断和治疗的潜在生物学设计。此外，我们描述了为更快速和更安全地临床使用工程系统而必须克服的挑战。合成生物学使工程细胞的发展成为可能，这些细胞可以作为体外或体内的诊断工具或治疗输送系统。这篇综述讨论了细菌和哺乳动物嗅觉细胞的临床前和临床应用，以及它们潜在的生物学设计和成功临床应用的仍然存在的障碍。
Synthetic biology seeks to redesign biological systems to perform novel functions in a predictable manner. Recent advances in bacterial and mammalian cell engineering include the development of cells that function in biological samples or within the body as minimally invasive diagnostics or theranostics for the real-time regulation of complex diseased states. Ex vivo and in vivo cell-based biosensors and therapeutics have been developed to target a wide range of diseases including cancer, microbiome dysbiosis and autoimmune and metabolic diseases. While probiotic therapies have advanced to clinical trials, chimeric antigen receptor (CAR) T cell therapies have received regulatory approval, exemplifying the clinical potential of cellular therapies. This Review discusses preclinical and clinical applications of bacterial and mammalian sensing and drug delivery platforms as well as the underlying biological designs that could enable new classes of cell diagnostics and therapeutics. Additionally, we describe challenges that must be overcome for more rapid and safer clinical use of engineered systems. Synthetic biology has enabled the development of engineered cells that can serve as ex vivo or in vivo diagnostic tools or therapeutic delivery systems. This Review discusses preclinical and clinical applications of bacterial and mammalian theranostic cells as well as their underlying biological designs and remaining hurdles to their successful clinical application.
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影响因子：
12.8
作者：
Cartellieri M;Feldmann A;Koristka S;Arndt C;Loff S;Ehninger A;von Bonin M;Bejestani EP;Ehninger G;Bachmann MP
通讯作者：
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影响因子：
46.9
作者：
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作者：
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通讯作者：
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影响因子：
16.6
作者：
Cho JH;Okuma A;Sofjan K;Lee S;Collins JJ;Wong WW
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影响因子：
16.6
作者：
Chassin H;Geering B;Schukur L;Ausländer D;Lang B;Fussenegger M
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