Relaxation effects of ferucarbotran-labeled mesenchymal stem cells at 1.5T and 3T: discrimination of viable from lysed cells.
Relaxation effects of ferucarbotran-labeled mesenchymal stem cells at 1.5T and 3T: discrimination of viable from lysed cells.
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DOI：
10.1002/mrm.22011
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发表时间：
2009-08
影响因子：
3.3
通讯作者：
Daldrup-Link, Heike
中科院分区：
文献类型：
--
作者：
Henning, Tobias D.;Wendland, Michael F.;Golovko, Daniel;Sutton, Elizabeth J.;Sennino, Barbara;Malek, Farbod;Bauer, Jan S.;McDonald, Donald M.;Daldrup-Link, Heike
通过简单孵育用Ferucarbotran标记人间充质干细胞（hMSC）并培养长达14天。通过光谱法测定铁含量，并通过电子显微镜和共聚焦显微镜研究造影剂摄取的细胞内定位。在标记后的不同时间点，达到1至14天，具有活的或裂解的标记的hMSC的样品以及未标记的对照进行MR成像。在临床扫描仪上，在1.5T和3 T下使用SE和GE序列以及多个TR和TE。光谱法显示每个细胞的初始氧化铁摄取为7.08 pg。显微镜研究显示溶酶体区室化。hMSC的造影剂效应在标记后持续长达14天。一个显着的差异，在T2-效应区室化的铁氧化物相比，游离铁氧化物被发现在T2加权序列，但不是T2* 序列。所观察到的差异可以解释由损失的分区的氧化铁颗粒，分布的均匀性和随后增加的退相的质子在SE图像。这些结果表明，具有区室化氧化铁的活细胞原则上可以与裂解细胞或释放的氧化铁区分开。
Human mesenchymal stem cells (hMSC) were labeled with Ferucarbotran by simple incubation and cultured for up to 14 days. Iron content was determined by spectrometry and the intracellular localization of the contrast agent uptake was studied by electron and confocal microscopy. At various time points after labeling, reaching from 1 to 14 days, samples with viable or lysed labeled hMSCs, as well as non-labeled controls underwent MR imaging. SE- and GE-sequences with multiple TRs and TEs were used at 1.5T and 3T on a clinical scanner. Spectrometry showed an initial iron oxide uptake of 7.08 pg per cell. Microscopy studies revealed lysosomal compartmentalization. Contrast agent effects of hMSC were persistent for up to 14 days after labeling. A marked difference in the T2-effect of compartmentalized iron oxides compared to free iron oxides was found on T2-weighted sequences, but not on T2*-sequences. The observed differences may be explained by the loss of compartmentalization of iron oxide particles, the uniformity of distribution and the subsequent increase in dephasing of protons on SE images. These results show that viable cells with compartmentalized iron oxides may – in principle – be distinguished from lysed cells or released iron oxides.
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影响因子：
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