The detection of wt-1 transcripts is not associated with an increased leukemic relapse rate in patients with acute leukemia after allogeneic bone marrow or peripheral blood stem cell transplantation
The detection of wt-1 transcripts is not associated with an increased leukemic relapse rate in patients with acute leukemia after allogeneic bone marrow or peripheral blood stem cell transplantation
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wt-1转录本的检测与异体骨髓或外周血干细胞移植后急性白血病患者白血病复发率增加无关
DOI：
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发表时间：
2000
影响因子：
4.8
通讯作者：
U. Schaefer
中科院分区：
文献类型：
--
作者：
A. Elmaagacli;D. Beelen;R. Trenschel;U. Schaefer
我们研究了46例急性白血病（AL）患者（第一次CR n = 24;第二次CR n = 9，复发n = 13）异基因骨髓或外周血干细胞移植后wt-1作为微小残留病（MRD）标志物的作用。在同种异体移植前，46例AL患者中有38例（83%）通过PCR检测到wt-1转录本。移植后，38例患者中有14例（37%）在移植后12个月（中位数1-89个月）的至少一次PCR检测中检测到wt-1转录本。38名患者中有12人在移植后复发，但12人中只有7人在移植后wt-1阳性。在5例复发患者中，wt-1试验在复发前0至3个月保持阴性。另一方面，移植后wt-1检测呈阳性的14名患者中只有7名连续复发。在46例研究患者中，有17例在移植前发现染色体异常（AML-M4 eo伴inv 16 n = 7，AML-M2伴t（8;21）n = 3，AML-M3伴t（15;17）n = 1，AML-M5伴t（4;11）n = 1，ALL伴t（9;22）n = 5）。在这17例患者中，我们分析了野生型1转录本的同时，一个特定的嵌合体转录本特征相应的染色体异常。在45个样本中的32个（71%）中，MRD标记物和wt-1转录物的结果一致，但在13个患者中不同。我们的结论是，检测wt-1转录并不能可靠地预测白血病复发，因此不是一个合适的MRD标记物在急性白血病患者异基因骨髓或外周血干细胞移植后。骨髓移植（2000年）25，91-96。
We studied the role of wt-1 as a minimal residual disease (MRD) marker in 46 patients with acute leukemia (AL) (1st CR n = 24; 2nd CR n = 9, in relapse n = 13) after allogeneic bone marrow or peripheral blood stem cell transplantation. Prior to allogeneic transplant, wt-1 transcripts were detected by PCR in 38 of 46 patients (83%) with AL. After transplant, in 14 of 38 patients (37%) wt-1 transcripts were detected in at least one PCR assay at a median of 12 months post transplant (range 1–89 months). Twelve of the 38 patients relapsed after transplant, but only seven of the 12 were wt-1 positive after transplant. In five relapsing patients the wt-1 test remained negative 0 to 3 months prior to relapse. On the other hand, only seven of 14 patients with a positive test for wt-1 after transplant, relapsed consecutively. In 17 of the 46 study patients chromosomal abnormalities had been found prior to transplant (AML-M4eo with inv16 n = 7, AML-M2 with t(8;21) n = 3, AML-M3 with t(15;17) n = 1, AML-M5 with t(4;11) n = 1, ALL with t(9;22) n = 5). In these 17 patients, we analyzed the wt-1 transcript simultaneously with a specific chimeric transcript characteristic for the corresponding chromosomal abnormality. In 32 of 45 samples (71%) the results for the MRD marker and wt-1 transcript were concordant, but differed in 13 patients. We conclude that detection of wt-1 transcripts does not predict leukemic relapse reliably and is therefore not a suitable MRD marker in patients with acute leukemia after allogeneic BM or PBSC transplantation. Bone Marrow Transplantation (2000) 25, 91–96.
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DOI：
10.1210/mend.8.5.8058069
发表时间：
1994
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
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作者：
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CURRAN, T
影响因子：
56.9
作者：
MADDEN, SL;COOK, DM;RAUSCHER, FJ
通讯作者：
RAUSCHER, FJ
影响因子：
56.9
作者：
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DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Harrington,MA;Konicek,B;Song,A;Xia,XL;Fredericks,WJ;Rauscher3rd,FJ
通讯作者：
Rauscher3rd,FJ