Interaction between sugar and abscisic acid signalling during early seedling development in Arabidopsis.
Interaction between sugar and abscisic acid signalling during early seedling development in Arabidopsis.
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DOI：
10.1007/s11103-008-9308-6
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发表时间：
2008-05
影响因子：
5.1
通讯作者：
Smeekens, Sjef C. M.
中科院分区：
文献类型：
--
作者：
Dekkers, Bas J. W.;Schuurmans, Jolanda A. M. J.;Smeekens, Sjef C. M.
糖调节植物中的重要过程并影响许多基因的表达。拟南芥（拟南芥）突变体的糖敏感性改变的表征揭示了脱落酸（阿坝）信号在糖反应的功能。然而，糖信号和阿坝之间的确切相互作用是模糊的。因此，产生了具有组成型ABI 4表达（aba 2 -1/35 S：：ABI 4）的阿坝缺陷型植物。ABI 4表达的增强并没有拯救aba 2幼苗的葡萄糖不敏感（gin）表型，表明其他阿坝调节因子也是必不可少的。有趣的是，葡萄糖和阿坝处理的拟南芥种子触发萌发后幼苗发育停滞。葡萄糖抑制的幼苗具有耐旱表型，并显示出葡萄糖诱导的脱落酸不敏感3（ABI 3），ABI 5和晚期胚胎发生丰富（莱亚）基因的表达，使人联想到早期幼苗发育过程中的阿坝信号。ABI 3是ABA诱导的停滞的关键调节剂，并且这里显示ABI 3也在葡萄糖信号传导中起作用。多个abi 3等位基因具有葡萄糖不敏感（gin）表型，与其他已知的gin突变体相当。重要的是，abi 3背景下葡萄糖调控的基因表达受到干扰。此外，abi 3在萌发过程中对糖不敏感，并表现出糖不敏感（sis）和蔗糖解偶联（sun）表型。突变体分析进一步鉴定了阿坝反应途径基因增强对ABA 1（ERA 1）和ABI 2作为葡萄糖信号传导的中间体。因此，三个以前未鉴定的糖信号基因已被确定，表明阿坝和葡萄糖信号重叠到一个更大的程度比原来认为的。
Sugars regulate important processes and affect the expression of many genes in plants. Characterization of Arabidopsis (Arabidopsis thaliana) mutants with altered sugar sensitivity revealed the function of abscisic acid (ABA) signalling in sugar responses. However, the exact interaction between sugar signalling and ABA is obscure. Therefore ABA deficient plants with constitutive ABI4 expression (aba2-1/35S::ABI4) were generated. Enhanced ABI4 expression did not rescue the glucose insensitive (gin) phenotype of aba2 seedlings indicating that other ABA regulated factors are essential as well. Interestingly, both glucose and ABA treatment of Arabidopsis seeds trigger a post-germination seedling developmental arrest. The glucose-arrested seedlings had a drought tolerant phenotype and showed glucose-induced expression of ABSCISIC ACID INSENSITIVE3 (ABI3), ABI5 and LATE EMBRYOGENESIS ABUNDANT (LEA) genes reminiscent of ABA signalling during early seedling development. ABI3 is a key regulator of the ABA-induced arrest and it is shown here that ABI3 functions in glucose signalling as well. Multiple abi3 alleles have a glucose insensitive (gin) phenotype comparable to that of other known gin mutants. Importantly, glucose-regulated gene expression is disturbed in the abi3 background. Moreover, abi3 was insensitive to sugars during germination and showed sugar insensitive (sis) and sucrose uncoupled (sun) phenotypes. Mutant analysis further identified the ABA response pathway genes ENHANCED RESPONSE TO ABA1 (ERA1) and ABI2 as intermediates in glucose signalling. Hence, three previously unidentified sugar signalling genes have been identified, showing that ABA and glucose signalling overlap to a larger extend than originally thought.
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影响因子：
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作者：
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通讯作者：
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