Microfluidics-based electrospray ionization enhances the intrasource separation of lipid classes and extends identification of individual molecular species through multi-dimensional mass spectrometry: development of an automated high-throughput platform for shotgun lipidomics.
Microfluidics-based electrospray ionization enhances the intrasource separation of lipid classes and extends identification of individual molecular species through multi-dimensional mass spectrometry: development of an automated high-throughput platform for shotgun lipidomics.
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DOI：
10.1002/rcm.3595
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发表时间：
2008-07
影响因子：
2
通讯作者：
Gross, Richard W.
中科院分区：
文献类型：
--
作者：
Han, Xianlin;Yang, Kui;Gross, Richard W.
在此，我们利用微流体和优化的泰勒锥的使用，用于在电喷雾电离过程中改善脂质类的胞内分离/选择性电离。通过具有基于芯片的电离的微流体技术实现了多种磷脂类的增加的差分电离，从而与常规离子源相比显著增强了磷脂类的胞内分离/选择性电离。例如，使用心肌脂质提取物，在输注样品溶液中不存在LiOH或其他碱性改性剂的情况下，在负离子模式下常规实现心肌磷脂类的膜内分离/选择性电离的3倍改善。重要的是，对应于每个脂质类中的单个分子种类的离子与从心肌提取物中选择的内标物的相对比率在基于芯片的界面和双稳态泵驱动的毛细血管界面之间几乎相同。因此，通过与各脂质类别中的内标物进行比较，直接从生物提取物中定量单个脂质分子种类很容易实现，其准确度和动态范围几乎与使用成熟的直接双螺杆泵驱动毛细管接口记录的准确度和动态范围相同。总的来说，与常规毛细管接口相比，微流体和机器人样品处理的使用显著增强了脂质的胞内分离，并且极大地促进了使用鸟枪脂质组学的多维质谱法的使用，从而为细胞脂质组的全局分析提供了自动化和高通量平台。
Herein, we exploit the use of microfluidics and optimized Taylor cones for improved intrasource separation/selective ionization of lipid classes during electrospray ionization. Increased differential ionization of multiple phospholipid classes was achieved through microfluidics with chip-based ionization resulting in substantial enhancement of intrasource separation/selective ionization of phospholipid classes in comparison to the conventional ion source. For example, using myocardial lipid extracts, 3-fold improvements in intrasource separation/selective ionization of myocardial phospholipid classes were routinely realized in the negative-ion mode in the absence of LiOH or other basic modifiers in the infused sample solutions. Importantly, the relative ratios of ions corresponding to individual molecular species in each lipid class to a selected internal standard from myocardial extracts were nearly identical between the chip-based interface and the syringe-pump-driven capillary interface. Therefore, quantitation of individual lipid molecular species directly from biological extracts through comparisons with internal standards in each lipid class was readily accomplished with an accuracy and dynamic range nearly identical to those documented using the well-established direct syringe-pump-driven capillary interface. Collectively, the use of microfluidics and robotic sample handling substantially enhances intrasource separation of lipids in comparison to routine capillary interfaces and greatly facilitates the use of multi-dimensional mass spectrometry using shotgun lipidomics, thereby providing an automated and high-throughput platform for global analyses of cellular lipidomes.
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影响因子：
2.9
作者：
Han, XL;GubitosiKlug, RA;Gross, RW
通讯作者：
Gross, RW
影响因子：
2.9
作者：
Jiang, Xuntian;Cheng, Hua;Han, Xianlin
通讯作者：
Han, Xianlin
影响因子：
2.9
作者：
Han, Xianlin;Yang, Jingyue;Gross, Richard W.
通讯作者：
Gross, Richard W.
DOI：
10.1016/j.jasms.2005.11.003
发表时间：
2006-02-01
影响因子：
3.2
作者：
Han, XL;Yang, K;Gross, RW
通讯作者：
Gross, RW
影响因子：
2.3
作者：
Ejsing, CS;Moehring, T;Shevchenko, A
通讯作者：
Shevchenko, A