Comparative Study of Pressure-Induced Germination of Bacillus subtilis Spores at Low and High Pressures
Comparative Study of Pressure-Induced Germination of Bacillus subtilis Spores at Low and High Pressures
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枯草芽孢杆菌孢子低压和高压压力诱导萌发的比较研究
DOI：
10.1128/aem.64.9.3220-3224.1998
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发表时间：
1998
影响因子：
4.4
通讯作者：
C. Michiels
中科院分区：
文献类型：
--
作者：
Elke Y. Wuytack;Steven Boven;C. Michiels
摘要我们研究了枯草芽孢杆菌孢子在中等压力（100 MPa）和高压力（500 ~ 600 MPa）下的压力诱导萌发。尽管我们通过使用热敏感性作为萌发标准发现两种条件下的萌发效率相当，但发现压力萌发孢子对一些其他试剂的敏感性取决于所使用的压力。在100 MPa下萌发的孢子比在600 MPa下萌发的孢子对压力（>200 MPa）、UV光和过氧化氢更敏感。由于小，酸溶性蛋白质（SASP）和吡啶二羧酸（DPA）是已知的参与孢子抗紫外线和过氧化氢，我们研究了这些化合物在压力发芽的命运。DPA被释放后，低压和高压发芽，但SASP降解，这通常伴随着营养诱导的发芽，发生在低压发芽，但不是在高压发芽。这些结果充分解释了在600 MPa下萌发的孢子的UV和过氧化氢抗性。萌发孢子对600 MPa压力失活的抗性也可以至少部分归因于α/β型SASPs，因为α/β型SASPs缺陷的突变体对600 MPa下的失活更敏感。此外，发芽在100 MPa导致快速ATP的产生，是在营养诱导发芽的情况下，但没有ATP在600 MPa发芽过程中形成。这些结果表明，孢子萌发可以启动低压和高压处理，但在后一种情况下，在早期阶段被逮捕。使用高压作为保存处理的影响进行了讨论。
ABSTRACT We have studied pressure-induced germination of Bacillus subtilis spores at moderate (100 MPa) and high (500 to 600 MPa) pressures. Although we found comparable germination efficiencies under both conditions by using heat sensitivity as a criterion for germination, the sensitivity of pressure-germinated spores to some other agents was found to depend on the pressure used. Spores germinated at 100 MPa were more sensitive to pressure (>200 MPa), UV light, and hydrogen peroxide than were those germinated at 600 MPa. Since small, acid-soluble proteins (SASPs) and dipicolinic acid (DPA) are known to be involved in spore resistance to UV light and hydrogen peroxide, we studied the fate of these compounds during pressure germination. DPA was released upon both low- and high-pressure germination, but SASP degradation, which normally accompanies nutrient-induced germination, occurred upon low-pressure germination but not upon high-pressure germination. These results adequately explain the UV and hydrogen peroxide resistance of spores germinated at 600 MPa. The resistance to pressure inactivation of 600-MPa-germinated spores could also, at least partly, be attributed to α/β-type SASPs, since mutants deficient in α/β-type SASPs were more sensitive to inactivation at 600 MPa. Further, germination at 100 MPa resulted in rapid ATP generation, as is the case in nutrient-induced germination, but no ATP was formed during germination at 600 MPa. These results suggest that spore germination can be initiated by low- and high-pressure treatments but is arrested at an early stage in the latter case. The implications for the use of high pressure as a preservation treatment are discussed.
DOI：
10.1111/j.1365-2672.1994.tb04357.x
发表时间：
1994-01-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
SETLOW, P
通讯作者：
SETLOW, P