New-generation screening assays for the detection of anti-influenza compounds targeting viral and host functions.
New-generation screening assays for the detection of anti-influenza compounds targeting viral and host functions.
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DOI：
10.1016/j.antiviral.2013.07.018
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发表时间：
2013-10
影响因子：
7.6
通讯作者：
Shaw, Megan L.
中科院分区：
文献类型：
--
作者：
Beyleveld, Grant;White, Kris M.;Ayllon, Juan;Shaw, Megan L.
关键词：
目前流感抗病毒治疗的选择仅限于神经氨酸酶抑制剂，并且在先前流行的H1N1病毒中已经观察到高水平的奥司他韦耐药性的知识增加了寻找新的流感治疗剂的紧迫性。为了满足这一需求，目前正在开发适用于高通量筛选的检测方法，并在本文中进行了讨论。特别强调的是基于细胞的测定，捕获病毒功能的抑制剂以及促进最佳流感病毒复制的宿主功能。这一领域的成功得益于对流感病毒基因组结构的更深入了解以及产生携带报告基因的可复制重组病毒的能力，从而允许在高通量环境中轻松监测病毒感染。这篇文章是抗病毒研究专题讨论会的一部分，主题是“治疗流感：靶向病毒或宿主”。
Current options for influenza antiviral therapy are limited to the neuraminidase inhibitors, and knowledge that high levels of oseltamivir resistance have been seen amongst previously circulating H1N1 viruses increases the urgency to find new influenza therapeutics. To feed this pipeline, assays that are appropriate for use in high-throughput screens are being developed and are discussed in this review. Particular emphasis is placed on cell-based assays that capture both inhibitors of viral functions as well as the host functions that facilitate optimal influenza virus replication. Success in this area has been fueled by a greater understanding of the genome structure of influenza viruses and the ability to generate replication-competent recombinant viruses that carry a reporter gene, allowing for easy monitoring of viral infection in a high-throughput setting. This article forms part of a symposium in Antiviral Research on “Treatment of influenza: targeting the virus or the host.”
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