Retinoic acid reduces the yield of herpes simplex virus in Vero cells and alters the N-glycosylation of viral envelope proteins.
Retinoic acid reduces the yield of herpes simplex virus in Vero cells and alters the N-glycosylation of viral envelope proteins.
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视黄酸可降低 Vero 细胞中单纯疱疹病毒的产量并改变病毒包膜蛋白的 N-糖基化。
DOI：
10.1016/s0166-3542(00)00090-5
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发表时间：
2000
影响因子：
7.6
通讯作者：
Kascsak,R
中科院分区：
文献类型：
--
作者：
Isaacs,CE;Xu,W;Pullarkat,RK;Kascsak,R
用全反式维甲酸（RA）处理Vero细胞，与对照培养物相比，感染性单纯疱疹病毒（HSV）的产生减少了1000- 10000倍。 RA处理后产生的总HSV包膜糖蛋白gB、gC和gD的水平与对照培养物中发现的水平相当。RA处理24小时后，除了对照样品中发现的每种蛋白质的典型分子量外，还产生了gB、gC和gD的较低分子量变体。在RA治疗的24和48小时之间，低分子量变体的比例增加。当对照和RA处理的样品与肽N-糖苷酶F（PNGase F）（其去除N-糖基化糖）孵育时，两组中产生的相应gB、gC和gD蛋白的分子量相当，表明RA在蛋白合成期间不改变病毒蛋白的一级序列或增加病毒蛋白的蛋白水解。RA治疗增加[3 H]甘露糖掺入HSV感染细胞的糖蛋白，但没有改变[3 H]葡糖胺掺入。我们的结论是，RA治疗不减少三个主要的病毒包膜糖蛋白的合成，但改变了它们的N-糖基化，并假设RA的抑制作用与其对N-糖基化的作用有关。
Treatment of Vero cells with all-trans-retinoic acid (RA) decreased the production of infectious herpes simplex virus (HSV) by 1000–10 000-fold when compared with control cultures. Levels of total HSV envelope glycoproteins gB, gC and gD produced following RA treatment, were comparable with those found in control cultures. Following 24 h of RA treatment, lower molecular weight variants of gB, gC and gD were produced in addition to the typical molecular mass of each protein found in control samples. Between 24 and 48 h of RA treatment, the proportion of the lower molecular mass variants increased. When control and RA treated samples were incubated with peptide N-glycosidase F (PNGase F), which removes N-glycosylated sugars, the molecular weights of the respective gB, gC and gD proteins produced were comparable in both the groups, indicating that RA did not alter the primary sequence of viral proteins during protein synthesis or increase viral protein proteolysis. RA treatment increased [3H]mannose incorporation into glycoproteins in HSV infected cells but did not change [3H]glucosamine incorporation. We conclude that RA treatment does not reduce the synthesis of three major viral envelope glycoproteins but alters their N-glycosylation and postulate that the inhibitory effect of RA is related to its action on N-glycosylation.
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类固醇激素受体配体对蛋白质的共价修饰。
DOI：
--
发表时间：
1992
影响因子：
11.1
作者：
N. Takahashi;T. Breitman
通讯作者：
T. Breitman
钙连接蛋白与 1 型单纯疱疹病毒糖蛋白 B、C 和 D 的前体相关。
影响因子：
3.7
作者：
Y. Yamashita;M. Yamada;T. Daikoku;H. Yamada;A. Tadauchi;T. Tsurumi;Y. Nishiyama
通讯作者：
Y. Nishiyama
高尔基系统糖基转移酶缺陷细胞中单纯疱疹病毒 1 聚糖的加工：与细胞融合和病毒颗粒排出的关系。
影响因子：
3.7
作者：
F. Serafini‐Cessi;F. dall’Olio;M. Scannavini;G. Campadelli
通讯作者：
G. Campadelli
实验性疱疹性角膜炎中的维生素 A。
DOI：
--
发表时间：
1981
期刊：
A M A Archives of Ophthalmology
影响因子：
--
作者：
M. Starr;C. Dawson;O. Briones;J. Oh
通讯作者：
J. Oh
影响因子：
56.9
作者：
HAMMOND, C;HELENIUS, A
通讯作者：
HELENIUS, A