The Vibrio vulnificus stressosome is an oxygen-sensor involved in regulating iron metabolism.
The Vibrio vulnificus stressosome is an oxygen-sensor involved in regulating iron metabolism.
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DOI：
10.1038/s42003-022-03548-w
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发表时间：
2022-06-27
影响因子：
5.9
通讯作者：
中科院分区：
文献类型：
--
作者：
压力体是细菌中广泛保守的压力感应蛋白复合物。虽然在革兰氏阳性菌中的一般应激反应的调节中的作用是有据可查的，但激活信号仍然不清楚，并且对革兰氏阴性菌中的应激体的生理功能知之甚少。本文对革兰氏阴性海洋病原菌创伤弧菌的应激体进行了研究。我们证明，它的感觉氧，并确定其在调节铁代谢的作用。我们确定了冷冻电子显微镜结构的VvRsbR：VvRsbS应激体复合物，第一个解决了从革兰氏阴性细菌。该结构指向VvRsbR和VvRsbS化学计量的变化和VvRsbR的氧传感域中的对称性破坏，这表明信号传感是如何激发应激反应的。这些发现提供了配体依赖性信号传导和输出之间的联系-铁代谢的调节-对于应激体复合物。一个cryo-electron显微镜重建的应激体复杂的革兰氏阴性细菌，创伤弧菌，揭示了亚基组成和对称性的变化，这可能有助于调整激活阈值的响应低水平的氧气和饥饿。
Stressosomes are stress-sensing protein complexes widely conserved among bacteria. Although a role in the regulation of the general stress response is well documented in Gram-positive bacteria, the activating signals are still unclear, and little is known about the physiological function of stressosomes in the Gram-negative bacteria. Here we investigated the stressosome of the Gram-negative marine pathogen Vibrio vulnificus. We demonstrate that it senses oxygen and identified its role in modulating iron-metabolism. We determined a cryo-electron microscopy structure of the VvRsbR:VvRsbS stressosome complex, the first solved from a Gram-negative bacterium. The structure points to a variation in the VvRsbR and VvRsbS stoichiometry and a symmetry breach in the oxygen sensing domain of VvRsbR, suggesting how signal-sensing elicits a stress response. The findings provide a link between ligand-dependent signaling and an output – regulation of iron metabolism - for a stressosome complex. A cryo-electron microscopy reconstruction of a stressosome complex from a Gram-negative bacterium, Vibrio vulnificus, reveals variations in subunit composition and symmetry, which could serve to adjust the activation threshold in the response to low levels of oxygen and starvation.
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影响因子：
16.6
作者：
Jia X;Wang JB;Rivera S;Duong D;Weinert EE
通讯作者：
Weinert EE
影响因子：
3.4
作者：
Blankenburg, Sascha;Hentschker, Christian;Voelker, Uwe
通讯作者：
Voelker, Uwe
影响因子：
3
作者：
de la Rosa-Trevin, J. M.;Quintana, A.;Carazo, J. M.
通讯作者：
Carazo, J. M.
影响因子：
16.8
作者：
Boël G;Smith PC;Ning W;Englander MT;Chen B;Hashem Y;Testa AJ;Fischer JJ;Wieden HJ;Frank J;Gonzalez RL Jr;Hunt JF
通讯作者：
Hunt JF
影响因子：
3.6
作者：
Chen, CC;Lewis, RJ;Delumeau, O
通讯作者：
Delumeau, O