Long non-coding RNAs as surrogate indicators for chemical stress responses in human-induced pluripotent stem cells.
Long non-coding RNAs as surrogate indicators for chemical stress responses in human-induced pluripotent stem cells.
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DOI：
10.1371/journal.pone.0106282
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Torimura M
中科院分区：
文献类型：
--
作者：
Tani H;Onuma Y;Ito Y;Torimura M
在这项研究中，我们重点研究了两种作为毒理学评估理想工具的生物制品：长链非编码rna （lncRNAs）和人类诱导的多能干细胞（hiPSCs）。lncrna是一类重要的普遍存在的非蛋白编码转录物，参与细胞应激反应的分子机制。hipsc具有自我更新和分化为多种细胞类型的能力，并且它们不存在与人类胚胎干细胞相关的伦理问题。在这里，我们鉴定了六个新的lncrna (CDKN2B-AS1, MIR22HG, GABPB1-AS1, FLJ33630， LINC00152和LINC0541471_v2)，它们在hiPSCs中响应模型化学应激（环己亚胺，过氧化氢，镉或砷）。我们的研究结果表明，lncrna对一般和特定的化学胁迫有反应。与p53相关mrna等典型mrna相比，lncRNAs对化学胁迫的反应高且快速。我们认为这些lncrna有潜力成为hipsc中化学应激反应的替代指标。
In this study, we focused on two biological products as ideal tools for toxicological assessment: long non-coding RNAs (lncRNAs) and human-induced pluripotent stem cells (hiPSCs). lncRNAs are an important class of pervasive non-protein-coding transcripts involved in the molecular mechanisms associated with responses to cellular stresses. hiPSCs possess the capabilities of self-renewal and differentiation into multiple cell types, and they are free of the ethical issues associated with human embryonic stem cells. Here, we identified six novel lncRNAs (CDKN2B-AS1, MIR22HG, GABPB1-AS1, FLJ33630, LINC00152, and LINC0541471_v2) that respond to model chemical stresses (cycloheximide, hydrogen peroxide, cadmium, or arsenic) in hiPSCs. Our results indicated that the lncRNAs responded to general and specific chemical stresses. Compared with typical mRNAs such as p53-related mRNAs, the lncRNAs highly and rapidly responded to chemical stresses. We propose that these lncRNAs have the potential to be surrogate indicators of chemical stress responses in hiPSCs.
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影响因子：
64.5
作者：
Huarte M;Guttman M;Feldser D;Garber M;Koziol MJ;Kenzelmann-Broz D;Khalil AM;Zuk O;Amit I;Rabani M;Attardi LD;Regev A;Lander ES;Jacks T;Rinn JL
通讯作者：
Rinn JL
影响因子：
7
作者：
Tani H;Mizutani R;Salam KA;Tano K;Ijiri K;Wakamatsu A;Isogai T;Suzuki Y;Akimitsu N
通讯作者：
Akimitsu N
影响因子：
3.7
作者：
Tani H;Torimura M;Akimitsu N
通讯作者：
Akimitsu N
DOI：
10.1016/j.bbrc.2013.09.006
发表时间：
2013-10-04
影响因子：
3.1
作者：
Tani, Hidenori;Torimura, Masaki
通讯作者：
Torimura, Masaki
影响因子：
64.8
作者：
Park, In-Hyun;Zhao, Rui;Daley, George Q.
通讯作者：
Daley, George Q.