Antioxidants Potential of the Filamentous Fungi (Mucor circinelloides).
Antioxidants Potential of the Filamentous Fungi (Mucor circinelloides).
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丝状真菌（卷枝毛霉）的抗氧化潜力
DOI：
10.3390/nu9101101
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发表时间：
2017-10-07
期刊：
影响因子：
5.9
通讯作者：
Song Y
中科院分区：
文献类型：
--
作者：
Hameed A;Hussain SA;Yang J;Ijaz MU;Liu Q;Suleria HAR;Song Y
在深层发酵条件下，将毛霉属的三个重要菌株在完全培养基和基本培养基中培养特定时间（72小时、120小时和168小时），研究其产生潜在抗氧化剂/次生代谢产物的能力。所有菌丝体提取物在β - 胡萝卜素/亚油酸漂白、自由基清除、金属离子还原以及对亚铁离子的螯合能力方面均表现出有效的抗氧化活性。不同的提取方法和溶剂系统对提取物的回收率和抗氧化活性有显著影响（p≤0.05）。乙醇提取物被发现是抗氧化成分的丰富来源，因此在抗氧化性能方面更有效。发酵时间和所用培养基也对抗氧化剂的产生和由此产生的抗氧化性能有显著影响（p≤0.05）。所有菌株在指数生长后期（120小时）的（乙醇）提取物显示出最高的抗氧化剂产量，具有最强的还原、螯合和自由基清除能力。发现菌株MC277.49是抗氧化剂产量最高的，其次是MC108.16和WJ11。酚类化合物的含量显著较高（p≤0.05），其次是缩合单宁和黄酮类化合物。每种提取物的总酚含量与总体抗氧化能力有关。发现具有营养胁迫条件的深层发酵是生产大量天然抗氧化剂/次生代谢产物的极好方法，它们在食品和制药工业中具有巨大的潜在商业应用。
Three important strains of Mucor circinelloides grown in complete and minimal media for specified period (72 h, 120 h and 168 h) under submerged fermentation conditions were investigated for their potential antioxidants/secondary metabolite production. All mycelial extracts demonstrated effective antioxidant activities in terms of β-carotene/linoleic acid bleaching, radical scavenging, reduction of metal ions and chelating abilities against ferrous ions. Different extraction methods and solvent systems affected the recovery yield and antioxidant activities of the extracts significantly (p ≤ 0.05). Ethanolic extracts were found to be rich source of antioxidant components and subsequently more effective in antioxidant properties. Fermentation period and media used also significantly affected (p ≤ 0.05) the antioxidant production and the resulting antioxidant properties. The (ethanolic) extracts of all the strains from late exponential growth phase (120 h) showed highest antioxidant production with topmost reducing, chelating and radical scavenging capabilities. Strain MC277.49 was found to be the highest producer of antioxidants followed by MC108.16 and WJ11. Phenolic compounds were detected significantly in higher (p ≤ 0.05) amount succeeded by the condensed tannins and flavonoids. Total phenol content of each extract was attributed to overall antioxidant capacity. Submerged fermentation with nutritional stress conditions were found to be excellent way of producing surplus amount of natural antioxidants/secondary metabolites with their vast potential commercial application in food and pharmaceutical industries.
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DOI：
10.3390/antiox5010006
发表时间：
2016-02-02
期刊：
Antioxidants (Basel, Switzerland)
影响因子：
--
作者：
Sugiharto S;Yudiarti T;Isroli I
通讯作者：
Isroli I
影响因子：
1.8
作者：
Apak, Resat;Gorinstein, Shela;Guclu, Kubilay
通讯作者：
Guclu, Kubilay
影响因子：
--
作者：
Mahapatra S;Banerjee D
通讯作者：
Banerjee D
影响因子：
5.4
作者：
Quettier-Deleu, C;Gressier, B;Trotin, F
通讯作者：
Trotin, F
DOI：
10.1111/j.1432-1033.1992.tb17334.x
发表时间：
1992-10-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
KENDRICK, A;RATLEDGE, C
通讯作者：
RATLEDGE, C