In vitro conditions for budded-to-hyphal-form transition of Trichosporon asahii of different sources
In vitro conditions for budded-to-hyphal-form transition of Trichosporon asahii of different sources
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不同来源 Asahii 毛孢子芽向菌丝形态转变的体外条件
DOI：
10.5897/ajmr11.958
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发表时间：
2012-01
期刊：
影响因子：
--
通讯作者：
杨蓉娅
中科院分区：
文献类型：
--
作者：
祝贺;王文岭;李海涛;敖俊红;杨蓉娅
目的研究阿萨希丝孢酵母菌丝型的转变。asahii）; 11株T.将asahii菌株分离并分别在RPMI-1640培养基、YPD液体培养基、Tween酵母肉汤（TYB）培养基和50%（v/v）胎牛血清（FBS）培养基中诱导菌丝生长。在15、25和37°C下进行培养1、2、3、6、12和24小时，并计算菌丝形成（生长）速率。在同一时间点，15 ℃、25 ℃和37 ℃下的平均菌丝形成率分别为（0.15±0.42）%、（5.75±3.48）%和（33.81±15.39）%（P均0.05）。平均菌丝形成率为在RPMI-1640中为（46.24±25.50）%，（36.28±21.85）%，TYB为（33.93±21.29）%，50%（v/v）FBS中的（18.60±14.58）%，组间（F = 6.29，P = 0.0013）和组内（F = 21.80，P = 0.0000）差异有统计学意义。此外，各菌株的平均菌丝形成率也有所提高。随着诱导时间的延长，在不同的菌丝诱导条件下，对T.在RBMI 1640液体培养基上，37°C培养24 h是获得高纯度菌丝的最佳条件。菌源也是影响菌丝生长的重要因素。朝平。
关键词：阿氏丝孢酵母，菌丝生长，诱导，培养基。
To investigate the hyphal-form transition of Trichosporon asahii (T. asahii) of different sources under different temperatures, media and cultural times; eleven T. asahii strains were isolated and maintained in RPMI-1640 medium, YPD liquid medium, Tween yeast bouillon (TYB) medium and 50% (v/v) fetal calf serum (FBS) medium independently to induce the hyphal growth. Culture was performed at 15, 25 and 37°C for 1, 2, 3, 6, 12 and 24 h and the hyphal formation (growth) rate was calculated. The mean hyphal formation rate was (0.15±0.42)% at 15, (5.75±3.48)% at 25°C and (33.81±15.39)% at 37°C (P<0.05 for all) at the same time point. The mean hyphal formation rate was (46.24±25.50)% in RPMI-1640, (36.28±21.85)% in YPD, (33.93±21.29)% in TYB and (18.60±14.58)% in 50%(v/v) FBS showing significant difference intergroups (F = 6.29, P = 0.0013) and intragroups (F = 21.80, P = 0.0000). Moreover, the mean hypha formation rate also raised in all T. asahiistrains with the prolongation of induction. Among various conditions for hypha induction ofT. asahii, culture in RBMI1640 liquid media at 37°C for 24 h is the best condition in which the hypha with high purity can be collected. The source of fungus is also an important factor influencing the hyphal growth of T. asahii.
Key words: Trichosporon asahii, hyphal growth, induction, medium.
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影响因子：
3.7
作者：
Martin R;Moran GP;Jacobsen ID;Heyken A;Domey J;Sullivan DJ;Kurzai O;Hube B
通讯作者：
Hube B
影响因子：
2.9
作者：
Takashi Sugita;A. Nishikawa;T. Ichikawa;Reiko Ikeda;T. Shinoda
通讯作者：
Takashi Sugita;A. Nishikawa;T. Ichikawa;Reiko Ikeda;T. Shinoda
影响因子：
1.6
作者：
Kumar S;Bandyopadhyay M;Mondal S;Pal N
通讯作者：
Pal N
DOI：
--
发表时间：
2007
期刊：
Chinese Journal of Nosocomiology
影响因子：
--
作者：
Wang Cong-min
通讯作者：
Wang Cong-min
影响因子：
--
作者：
Rongya Yang;J. Ao;Wen-ling Wang;Dawei Li;Ruoyu Li
通讯作者：
Rongya Yang;J. Ao;Wen-ling Wang;Dawei Li;Ruoyu Li