Specific protein binding to the simian virus 40 enhancer in vitro
Specific protein binding to the simian virus 40 enhancer in vitro
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体外与猿猴病毒 40 增强子结合的特异性蛋白
DOI：
10.1128/mcb.6.6.2098-2105.1986
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发表时间：
1986
影响因子：
5.3
通讯作者：
Keikichi Takahashi
中科院分区：
文献类型：
--
作者：
Alan Wildeman;T. M. Zenke;T. C. Schatz;Marguerite Wintzerith;Thomas;Grundstrom;Hans Matthes;Keikichi Takahashi
用HeLa细胞核提取液和野生型或突变的猴病毒40增强子DNA进行DNase I足迹实验，研究可能的反式作用因子与多个增强子基序的相互作用。我们发现这些核提取物含有与这些基序结合的蛋白质。由于在体内对特定增强子基序的活性有害的点突变专门阻止了该基序在体内对DNase I的消化的保护，我们认为结合的蛋白对应于参与转录增强的反式作用因子。利用突变体，其中猴病毒40增强子的两个结构域A和B要么通过插入DNA片段而分离，要么相对于它们的自然方向反向，我们还证明了反式作用因子独立地与这两个结构域结合。
HeLa cell nuclear extracts and wild-type or mutated simian virus 40 enhancer DNA were used in DNase I footprinting experiments to study the interaction of putative trans-acting factors with the multiple enhancer motifs. We show that these nuclear extracts contain proteins that bind to these motifs. Because point mutations which are detrimental to the activity of a particular enhancer motif in vivo specifically prevent protection of that motif against DNase I digestion in vivo, we suggest that the bound proteins correspond to trans-acting factors involved in enhancement of transcription. Using mutants in which the two domains A and B of the simian virus 40 enhancer are either separated by insertion of DNA fragments or inverted with respect to their natural orientation, we also demonstrate that the trans-acting factors bind independently to the two domains.
免疫球蛋白重链增强剂需要一种或多种组织特异性因子。
DOI：
10.1126/science.3917575
发表时间：
1985
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Mercola,M;Goverman,J;Mirell,C;Calame,K
通讯作者：
Calame,K