Reactive Oxygen Species Responsive Cleavable Hierarchical Metallic Supra-Nanostructure.
Reactive Oxygen Species Responsive Cleavable Hierarchical Metallic Supra-Nanostructure.
复制标题
活性氧响应可切割的分级金属超纳米结构。
DOI：
10.1002/smll.202202694
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发表时间：
2022-09
期刊：
影响因子：
13.3
通讯作者：
Kim, Dong-Hyun
中科院分区：
文献类型：
--
作者：
Choi, Hyunjun;Choi, Bongseo;Han, Jun-Hyeok;Shin, Ha Eun;Park, Wooram;Kim, Dong-Hyun
关键词：
我们报道了一种活性氧（ROS）响应可切割的分层金属超纳米结构（HMSN）。以金/银/胆酸盐为选择配比，采用一锅水法制备了由原生金（Au）纳米晶体和银（Ag）纳米连接体组成的细支结构的HMSN。在内源性和外源性活性氧存在下，测试了HMSN的活性氧响应降解性。在过氧化氢（H2O2）溶液中，观察到HMSN明显的ROS响应结构变形。与传统的球形金纳米颗粒相比，HMSN的活性氧响应性可忽略不计。在光动力疗法（PDT）或x射线辐射产生的各种细胞内源性ROS和外源性ROS的体外ROS条件下，进一步证实了HMSN的ROS响应性降解。然后，在小鼠肿瘤模型中，通过瘤内注射HMSN和PDT产生外源性ROS，进一步评估HMSN的体内ROS响应降解性。另外，研究了注射后30天静脉给药HMSN的体内生物分布和毒性，以研究其潜在的体内应用。观察到的HMSN的活性氧反应降解性将为一类活性氧反应多功能纳米载体在癌症治疗和各种生物医学应用中提供一个有希望的选择。本研究发现，在生物内源性活性氧（ROS）和外源性活性氧条件下，由原生金纳米分支与银纳米连接物组成的分层金属超纳米结构（HMSN）是可切割的。鉴于各种有效无机纳米颗粒的不可降解和非刺激响应性的挑战，ROS响应可切割HMSN的引入为ROS响应性纳米载体的应用提供了一个令人兴奋的机会。
We report a reactive oxygen species (ROS) responsive cleavable hierarchical metallic supra-nanostructure (HMSN). HMSN structured with thin branches composed by primary gold (Au) nanocrystals and silver (Ag) nano-linkers was synthesized by a one-pot aqueous synthesis with a selected ratio of Au/Ag/cholate. ROS responsive degradability of HMSN was tested in the presence of endogenous and exogeneous ROS. Significant ROS responsive structural deformation of HMSN was observed in the ROS exposure with hydrogen peroxide (H2O2) solution. The ROS responsiveness of HMSN was significantly comparable with negligible structural changes of conventional spherical gold nanoparticles. Demonstrated ROS responsive degradation of HMSN was further confirmed in various in vitro ROS conditions of each cellular endogenous ROS and exogeneous ROS generated by photodynamic therapy (PDT) or X-ray radiation. Then, in vivo ROS responsive degradability of HMSN was further evaluated with intratumoral injection of HMSN and exogeneous ROS generation via PDT in a mice tumor model. Additional in vivo biodistribution and toxicity of intravenously administrated HMSN at 30-day post injection were investigated for potential in vivo applications. Observed ROS responsive degradability of HMSN will provide a promising option for a type of ROS responsive-multifunctional nanocarriers in the cancer treatment and various biomedical applications. Here we found that hierarchical metallic supra-nanostructure (HMSN) composed by primary Au nanobranches connected with Ag nano-linkers was cleavable in the response to a biological endogenous reactive oxygen species (ROS) and exogeneous ROS condition. As given challenges of non-degradable and non-stimuli responsiveness of various potent inorganic nanoparticles, the introduction of ROS responsive cleavable HMSN suggests an exciting opportunity for the ROS-responsive nanocarrier applications.
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影响因子：
15
作者：
Park W;Park SJ;Cho S;Shin H;Jung YS;Lee B;Na K;Kim DH
通讯作者：
Kim DH
影响因子：
17.1
作者：
Poon, Wilson;Zhang, Yi-Nan;Chan, Warren C. W.
通讯作者：
Chan, Warren C. W.
影响因子：
16.6
作者：
Nowicka, Anna Maria;Hasse, Ulrich;Scholz, Fritz
通讯作者：
Scholz, Fritz
影响因子：
17.1
作者：
Choi, Bongseo;Choi, Hyunjun;Kim, Dong-Hyun
通讯作者：
Kim, Dong-Hyun
DOI：
10.1073/pnas.1911734116
发表时间：
2020-01-07
影响因子：
11.1
作者：
Balfourier, Alice;Luciani, Nathalie;Carn, Florent
通讯作者：
Carn, Florent