General Features and Novel Gene Signatures That Identify Epstein-Barr Virus-Associated Epithelial Cancers.
General Features and Novel Gene Signatures That Identify Epstein-Barr Virus-Associated Epithelial Cancers.
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DOI：
10.3390/cancers14010031
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发表时间：
2021-12-22
期刊：
影响因子：
5.2
通讯作者：
Ekalaksananan T
中科院分区：
文献类型：
--
作者：
Heawchaiyaphum C;Pientong C;Yoshiyama H;Iizasa H;Panthong W;Ekalaksananan T
Nasopharyngeal carcinoma (NPC), Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC), and oral squamous cell carcinoma (OSCC) are epithelial cancers that are associated with EBV infection. However, the gene signatures and common hallmarks associated with EBV infection in EBV-associated epithelial cancers (EBVaCAs) have not been fully elucidated. Here, we performed a panel of transcriptome analyses to identify the gene signatures and common hallmarks of these EBVaCAs. Based on the changes in the expression levels of genes in EBV-infected cell lines and tumor tissues, we identified two upregulated genes, SLC26A9 and TMC8, as gene signatures for EBVaCAs. In addition, SLC26A9 and TMC8 are differentially expressed genes (DEGs) in EBV-infected cells, and their expression is highly correlated with the stimulation of genes involved in numerous biological processes and pathways, such as IL6/JAK/STAT3 and TNF-α/NF-κB signaling pathways. Here, we propose SLC26A9 and TMC8 as novel gene signatures. In addition, we propose IL6/JAK/STAT3 and TNF-α/NF-κB signaling pathways as common hallmarks of EBVaCAs. Epstein-Barr virus (EBV) is associated with various types of human malignancies, including nasopharyngeal carcinoma (NPC), EBV-associated gastric carcinoma (EBVaGC), and oral squamous cell carcinoma (OSCC). The present study aimed to identify gene signatures and common signaling pathways that can be used to predict the prognosis of EBV-associated epithelial cancers (EBVaCAs) by performing an integrated bioinformatics analysis of cell lines and tumor tissues. We identified 12 differentially expressed genes (DEGs) in the EBVaCA cell lines. Among them, only four DEGs, including BAMBI, SLC26A9, SGPP2, and TMC8, were significantly upregulated. However, SLC26A9 and TMC8, but not BAMBI and SGPP2, were significantly upregulated in EBV-positive tumor tissues compared to EBV-negative tumor tissues. Next, we identified IL6/JAK/STAT3 and TNF-α/NF-κB signaling pathways as common hallmarks of EBVaCAs. The expression of key genes related to the two hallmarks was upregulated in both EBV-infected cell lines and EBV-positive tumor tissues. These results suggest that SLC26A9 and TMC8 might be gene signatures that can effectively predict the prognosis of EBVaCAs and provide new insights into the molecular mechanisms of EBV-driven epithelial cancers.
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影响因子：
3.8
作者：
Lu XQ;Zhang JQ;Zhang SX;Qiao J;Qiu MT;Liu XR;Chen XX;Gao C;Zhang HH
通讯作者：
Zhang HH
影响因子：
39.3
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Sun SC
影响因子：
11.2
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Heemers HV;Schmidt LJ;Sun Z;Regan KM;Anderson SK;Duncan K;Wang D;Liu S;Ballman KV;Tindall DJ
通讯作者：
Tindall DJ
影响因子：
4
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通讯作者：
Xiong B
影响因子：
5.4
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Frecha, Cecilia;Chevalier, Sebastien A.;Tommasino, Massimo
通讯作者：
Tommasino, Massimo