Hamster PIWI proteins bind to piRNAs with stage-specific size variations during oocyte maturation.
Hamster PIWI proteins bind to piRNAs with stage-specific size variations during oocyte maturation.
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DOI：
10.1093/nar/gkab059
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发表时间：
2021-03-18
影响因子：
14.9
通讯作者：
Siomi H
中科院分区：
文献类型：
--
作者：
Ishino K;Hasuwa H;Yoshimura J;Iwasaki YW;Nishihara H;Seki NM;Hirano T;Tsuchiya M;Ishizaki H;Masuda H;Kuramoto T;Saito K;Sakakibara Y;Toyoda A;Itoh T;Siomi MC;Morishita S;Siomi H
在动物性腺中，转座因子被主动抑制以通过PIWI相互作用RNA（piRNA）途径保持基因组完整性。在小鼠中，piRNA在雄性生殖细胞中大量表达，并与三种不同的PIWI形成效应复合物。单个Piwi基因的缺失导致雄性特异性不育，在雌性小鼠中没有可辨别的表型。与小鼠不同，大多数其他哺乳动物有四个PIWI基因，其中一些在卵巢中表达。在这里，从金黄仓鼠的卵母细胞纯化PIWI复合物揭示了PIWIL 1相关的piRNA的大小在卵母细胞成熟期间发生了变化。相比之下，PIWIL 3（大多数哺乳动物中的卵巢特异性PIWI）仅在中期II卵母细胞中与短piRNA缔合，这与蛋白质的强烈磷酸化一致。改进的高质量基因组组装和注释显示，PIWIL 1-和PIWIL 3-相关的piRNA似乎共享共同piRNA前体的5 ′-末端，并且主要来源于未注释的序列，TE-衍生序列的贡献减少，其中大部分对应于内源性逆转录病毒。我们的研究结果显示了仓鼠卵母细胞中piRNA生物发生的复杂性和动态性，并与产生的新基因组序列一起，作为开发有用模型以研究哺乳动物卵母细胞中piRNA途径的基础。
In animal gonads, transposable elements are actively repressed to preserve genome integrity through the PIWI-interacting RNA (piRNA) pathway. In mice, piRNAs are abundantly expressed in male germ cells, and form effector complexes with three distinct PIWIs. The depletion of individual Piwi genes causes male-specific sterility with no discernible phenotype in female mice. Unlike mice, most other mammals have four PIWI genes, some of which are expressed in the ovary. Here, purification of PIWI complexes from oocytes of the golden hamster revealed that the size of the PIWIL1-associated piRNAs changed during oocyte maturation. In contrast, PIWIL3, an ovary-specific PIWI in most mammals, associates with short piRNAs only in metaphase II oocytes, which coincides with intense phosphorylation of the protein. An improved high-quality genome assembly and annotation revealed that PIWIL1- and PIWIL3-associated piRNAs appear to share the 5′-ends of common piRNA precursors and are mostly derived from unannotated sequences with a diminished contribution from TE-derived sequences, most of which correspond to endogenous retroviruses. Our findings show the complex and dynamic nature of biogenesis of piRNAs in hamster oocytes, and together with the new genome sequence generated, serve as the foundation for developing useful models to study the piRNA pathway in mammalian oocytes.
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DOI：
10.1261/rna.045310.114
发表时间：
2014-08
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Hirano T;Iwasaki YW;Lin ZY;Imamura M;Seki NM;Sasaki E;Saito K;Okano H;Siomi MC;Siomi H
通讯作者：
Siomi H
影响因子：
4.9
作者：
Hancks DC;Kazazian HH Jr
通讯作者：
Kazazian HH Jr
影响因子：
48
作者：
Chin, Chen-Shan;Peluso, Paul;Schatz, Michael C.
通讯作者：
Schatz, Michael C.
模因套件：用于发现和搜索的工具。
影响因子：
14.9
作者：
Bailey TL;Boden M;Buske FA;Frith M;Grant CE;Clementi L;Ren J;Li WW;Noble WS
通讯作者：
Noble WS
影响因子：
64.5
作者：
Elkayam E;Kuhn CD;Tocilj A;Haase AD;Greene EM;Hannon GJ;Joshua-Tor L
通讯作者：
Joshua-Tor L