20S proteasomes secreted by the malaria parasite promote its growth.
20S proteasomes secreted by the malaria parasite promote its growth.
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DOI：
10.1038/s41467-021-21344-8
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发表时间：
2021-02-19
影响因子：
16.6
通讯作者：
Regev-Rudzki N
中科院分区：
文献类型：
--
作者：
Dekel E;Yaffe D;Rosenhek-Goldian I;Ben-Nissan G;Ofir-Birin Y;Morandi MI;Ziv T;Sisquella X;Pimentel MA;Nebl T;Kapp E;Ohana Daniel Y;Karam PA;Alfandari D;Rotkopf R;Malihi S;Temin TB;Mullick D;Revach OY;Rudik A;Gov NS;Azuri I;Porat Z;Bergamaschi G;Sorkin R;Wuite GJL;Avinoam O;Carvalho TG;Cohen SR;Sharon M;Regev-Rudzki N
Mature red blood cells (RBCs) lack internal organelles and canonical defense mechanisms, making them both a fascinating host cell, in general, and an intriguing choice for the deadly malaria parasite Plasmodium falciparum (Pf), in particular. Pf, while growing inside its natural host, the human RBC, secretes multipurpose extracellular vesicles (EVs), yet their influence on this essential host cell remains unknown. Here we demonstrate that Pf parasites, cultured in fresh human donor blood, secrete within such EVs assembled and functional 20S proteasome complexes (EV-20S). The EV-20S proteasomes modulate the mechanical properties of naïve human RBCs by remodeling their cytoskeletal network. Furthermore, we identify four degradation targets of the secreted 20S proteasome, the phosphorylated cytoskeletal proteins β-adducin, ankyrin-1, dematin and Epb4.1. Overall, our findings reveal a previously unknown 20S proteasome secretion mechanism employed by the human malaria parasite, which primes RBCs for parasite invasion by altering membrane stiffness, to facilitate malaria parasite growth. Plasmodium falciparum secretes extracellular vesicles (EVs) while growing inside red blood cells (RBCs). Here the authors show that these EVs contain assembled and functional 20S proteasome complexes that remodel the cytoskeleton of naïve human RBCs, priming the RBCs for parasite invasion.
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影响因子：
14.9
作者：
Gene Ontology Consortium
通讯作者：
Gene Ontology Consortium
影响因子：
2.8
作者：
Abdi, Abdirahman I.;Carvalho, Teresa G.;Doerig, Christian
通讯作者：
Doerig, Christian
影响因子：
5.5
作者：
Ben-Nissan G;Sharon M
通讯作者：
Sharon M
影响因子：
3.8
作者：
Celeste Nicolao, Maria;Rodriguez Rodrigues, Christian;Cumino, Andrea C.
通讯作者：
Cumino, Andrea C.
影响因子：
3.7
作者：
Darling AL;Uversky VN
通讯作者：
Uversky VN