Post-column reaction detectors for high-performance liquid chromatography

Post-column reaction detectors for high-performance liquid chromatography
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用于高效液相色谱的柱后反应检测器

DOI:
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发表时间:
1985
期刊:
影响因子:
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通讯作者:
U. Brinkman
U. Brinkman
中科院分区:
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文献类型:
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作者:
R. W. Frei;H. Jansen;U. Brinkman

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Currently, the best and most reliable HPLC (high-performance liquid chromatography) detectors are UV-VIS absorbance, fluorescence, and electrochemical detectors. It is attractive to try to expand their range of application by using suitable chemical derivatization techniques to convert the analytes of interest with their originally poor detection properties into compounds that can be detected with high sensitivity with these detectors. Besides an improvement of the detection properties, the chemical reaction can also enhance the selectivity of the total analytical method. The derivatization can be carried out either prior to the HPLC separation or by doing the reaction in an on-line postcolumn mode. Comparative advantages and disadvantages of these two approaches have been discussed previously. This paper will discuss on-line postcolumn derivatization. A general scheme of an HPLC system equipped with an on-line postcolumn reaction detector is given. 40 references, 6 figures, 2 tables.
DOI: 10.1016/0003-2697(82)90120-8
发表时间: 1982
影响因子: 2.9
作者:
Böhlen,P;Schroeder,R
通讯作者: Schroeder,R