Histological evaluation of interleukin-1 beta and collagen in gingival tissue from untreated adult periodontitis.
Histological evaluation of interleukin-1 beta and collagen in gingival tissue from untreated adult periodontitis.
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对未经治疗的成人牙周炎牙龈组织中白细胞介素 1 β 和胶原蛋白的组织学评估。
DOI：
10.1111/j.1600-0765.1994.tb01091.x
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发表时间：
1994
影响因子：
3.5
通讯作者：
J. Casey
中科院分区：
文献类型：
--
作者：
B. Feldner;R. Reinhardt;C. Garbin;G. Seymour;J. Casey
白细胞介素-1 β (IL-1 β)可能与牙周炎相关的病理过程有关，主要是因为它具有诱导胶原酶、增加中性粒细胞趋化性和刺激骨吸收的能力。本研究旨在对未经治疗的牙龈炎/早期牙周炎（G/EP）与中度/重度牙周炎（M/SP）牙龈组织中不同组织学领域的IL-1 β阳性细胞进行组织学定量，并将其与胶原流失联系起来。收集了8例患者的2份牙龈活检，1份来自G/EP部位，1份来自M/SP部位。采用小鼠单克隆抗体联合亲和素-生物素-过氧化物酶系统对IL-1 β进行染色，采用van Gieson法对连续切片进行胶原染色。G/EP区（35%）和M/SP区（52%）胶原蛋白损失与牙龈炎和牙周炎相一致。IL-1 β阳性细胞在冠状/管状区（Co/Su）和根尖/管状区（Ap/Su）（最靠近细菌损伤区）明显多于冠状/中区（Co/Mi）和根尖/中区（Ap/Mi） （p < 0.05）。虽然M/SP活检中IL-1 β阳性细胞的数量和百分比普遍较高，但差异不显著。此外，白细胞介素-1 β阳性细胞的数量与胶原蛋白损失百分比之间没有相关性。然而，IL-1 β阳性细胞与相应的龈沟液IL-1 β浓度显著相关（r = 0.65, p = 0.01）。通过使用免疫组织化学，本研究表明，IL-1 β +细胞的存在似乎与胶原蛋白损失没有直接关联。
Interleukin-1 beta (IL-1 beta) may be related to the pathological processes associated with periodontitis, primarily due to its ability to induce collagenase, increase neutrophil chemotaxis, and stimulate bone resorption. This study was designed to histologically quantitate IL-1 beta positive cells from various histologic fields in untreated gingivitis/early periodontitis (G/EP) versus moderate/severe periodontitis (M/SP) gingival tissues, and associate these with collagen loss. Two gingival biopsies from 8 patients were collected, one from a G/EP site and one from a M/SP site. Mouse monoclonal antibodies in combination with an avidin-biotin-peroxidase system were used to stain for IL-1 beta, while the van Gieson method was used to stain for collagen in serial sections. Collagen loss in G/EP (35%) and M/SP (52%) fields was consistent with gingivitis and periodontitis, respectively. IL-1 beta positive cells in combined coronal/sulcular (Co/Su) and apical/sulcular (Ap/Su) fields (nearest the bacterial insult) were significantly more numerous compared to combined coronal/middle (Co/Mi) and apical/middle (Ap/Mi) fields (p < 0.05). While numbers and percentages of IL-1 beta positive cells were generally higher in M/SP biopsies, differences were not significant. Further, there was no correlation between the number of IL-1 beta positive cells and percent collagen loss. However, a significant correlation between IL-1 beta positive cells and corresponding gingival crevicular fluid IL-1 beta concentrations was noted (r = 0.65, p = 0.01). Through the use of immunohistochemistry, this study demonstrated that the presence of IL-1 beta + cells does not appear to have a direct association with collagen loss.
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影响因子：
6.7
作者：
REINHARDT, RA;MASADA, MP;ALLISON, AC
通讯作者：
ALLISON, AC
影响因子：
6.7
作者：
Philip Stashenko;P. Fujiyoshi;M. Obernesser;L. Prostak;A. Haffajee;S. Socransky
通讯作者：
Philip Stashenko;P. Fujiyoshi;M. Obernesser;L. Prostak;A. Haffajee;S. Socransky
影响因子：
4.3
作者：
Passo,SA;Reinhardt,RA;DuBois,LM;Cohen,DM
通讯作者：
Cohen,DM
与牙龈出血相关的细胞群。
影响因子：
4.3
作者：
Cooper,PG;Caton,JG;Polson,AM
通讯作者：
Polson,AM
影响因子：
3.5
作者：
PAGE, RC
通讯作者：
PAGE, RC