Release of the ribosome biogenesis factor Bud23 from small subunit precursors in yeast.
Release of the ribosome biogenesis factor Bud23 from small subunit precursors in yeast.
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DOI：
10.1261/rna.079025.121
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Johnson AW
中科院分区：
文献类型：
--
作者：
Black JJ;Johnson AW
真核生物核糖体的两个亚基是通过准独立的途径产生的，这些途径涉及许多反式作用的生物发生因子的分级作用和核糖体蛋白的掺入。这些因素共同作用，通过一系列中间状态形成它们的功能结构，从而形成新生的亚单位。小亚基(SSU或40S)最早的中间体之一是SSU过程组，它随后被转化为40S前的中间体。这种转化在一定程度上是由甲基转移酶Bud23的结合促进的。Bud23是如何从生成的40岁前病毒中释放出来的还不清楚。核糖体蛋白Rps0、Rps2和Rps21称为Rps0-簇蛋白，几个生物发生因子在Bud23释放前后与前40个蛋白质结合，表明其中一个或多个因素可以诱导Bud23释放。在这里，我们系统地研究了这些因素对40s前颗粒释放Bud23的要求。我们发现Rps0-簇蛋白是Bud23释放所必需的，但不是充分的。非典型的激酶/ATPase RIO2与Bud23共享一个结合位点，被认为是在Rps0簇蛋白之后招募到40岁前。耗尽RIO2阻止了Bud23从40岁前释放。更重要的是，在从RIO2耗尽的细胞中亲和纯化的Pre-40s颗粒中加入重组RIO2，就足以在体外释放Bud23。RIO2取代Bud23的能力与核苷酸水解度无关。我们提出了RIO2的一个新角色，即它与40岁前的结合积极地取代了40岁前的Bud23。
The two subunits of the eukaryotic ribosome are produced through quasi-independent pathways involving the hierarchical actions of numerous trans-acting biogenesis factors and the incorporation of ribosomal proteins. The factors work together to shape the nascent subunits through a series of intermediate states into their functional architectures. One of the earliest intermediates of the small subunit (SSU or 40S) is the SSU processome which is subsequently transformed into the pre-40S intermediate. This transformation is, in part, facilitated by the binding of the methyltransferase Bud23. How Bud23 is released from the resultant pre-40S is not known. The ribosomal proteins Rps0, Rps2, and Rps21, termed the Rps0-cluster proteins, and several biogenesis factors bind the pre-40S around the time that Bud23 is released, suggesting that one or more of these factors could induce Bud23 release. Here, we systematically examined the requirement of these factors for the release of Bud23 from pre-40S particles. We found that the Rps0-cluster proteins are needed but not sufficient for Bud23 release. The atypical kinase/ATPase Rio2 shares a binding site with Bud23 and is thought to be recruited to pre-40S after the Rps0-cluster proteins. Depletion of Rio2 prevented the release of Bud23 from the pre-40S. More importantly, the addition of recombinant Rio2 to pre-40S particles affinity-purified from Rio2-depleted cells was sufficient for Bud23 release in vitro. The ability of Rio2 to displace Bud23 was independent of nucleotide hydrolysis. We propose a novel role for Rio2 in which its binding to the pre-40S actively displaces Bud23 from the pre-40S.
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DOI：
10.1083/jcb.202004161
发表时间：
2020-11-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Huang H;Ghalei H;Karbstein K
通讯作者：
Karbstein K
影响因子：
16
作者：
Grandi, P;Rybin, V;Hurt, E
通讯作者：
Hurt, E
影响因子：
0.8
作者：
Akhmetov, Azat;Laurent, Jon M.;Marcotte, Edward M.
通讯作者：
Marcotte, Edward M.
影响因子：
11.4
作者：
BRINK, MF;VERBEET, MP;DEBOER, HA
通讯作者：
DEBOER, HA
影响因子：
7.8
作者：
Korostelev, Andrei;Ermolenko, Dmitri N.;Noller, Harry F.
通讯作者：
Noller, Harry F.