Isolation and Identification of Two Algae-Lysing Bacteria against Microcystis aeruginosa
Isolation and Identification of Two Algae-Lysing Bacteria against Microcystis aeruginosa
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两种抗铜绿微囊藻溶藻菌的分离与鉴定
DOI：
10.3390/w12092485
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发表时间：
2020-09
期刊：
影响因子：
3.4
通讯作者：
Feng Jia
中科院分区：
文献类型：
--
作者：
Yang Jing;Qiao Kai;Lv Junping;Liu Qi;Nan Fangru;Xie Shulian;Feng Jia
藻类大量繁殖是一个全球性的环境问题。针对蓝藻水华暴发的问题，生物防治的作用引起了广泛关注，特别是溶藻细菌。在我们的研究中，确定了溶藻活性的机制。所用溶藻细菌是从太原市汾河风景区的水体和沉积物中分离得到的。采用基于16S rDNA基因的聚合酶链反应扩增进行遗传和分子鉴定。经鉴定，这两株细菌分别为植物拉乌尔氏菌（Raoultella planticola）和气单胞菌（Aeromonas sp.）。两种溶藻细菌的溶藻效果均与较高的接种率（>8%）有关。M.不同细胞龄的菌株均能完全去除铜绿假单胞菌。然而，所用的时间随着细胞年龄的增加而减少。M.铜绿假单胞菌处于滞后期和对数期。接种的时间越早，藻类的去除越快。这两种溶藻物质均为蛋白质，能破坏藻体的光合系统，使藻体细胞破裂。铜绿。本发明对经济可行的除藻具有重要的理论和实践意义，为蓝藻水华的控制提供了良好的种质资源和技术支持。
Algae blooms present an environmental problem worldwide. In response to the outbreak of harmful algal blooms in cyanobacteria, the role of biological control has drawn wide attention, particularly for algicidal bacteria. The mechanism underlying algicidal activity was determined in our study. Algae-lysing bacteria used were separated from water and sediment collected from the Fenhe scenic spot of Taiyuan. Genetic and molecular identification was conducted by polymerase chain reaction amplification based on 16S rDNA gene. These bacterial strains were identified as Raoultella planticola and Aeromonas sp. The algae-lysing characteristics were evaluated on Microcystis aeruginosa. For the two algicidal bacteria, the high inoculation ratio (>8%) of bacteria strains contributed to the lytic effect. M. aeruginosa could be completely removed by these strains at different cell ages. However, the time used decreased with an increase in cell age. The removal rate was increased while M. aeruginosa was in the lag and logarithmic phases. The earlier bacteria strains could be inoculated, the sooner all algae could be removed. Both algicidal substances were protein, which could destroy the photosynthetic systems and break the cell of M. aeruginosa. The algicidal bacteria strain has important theoretical and practical significance for economic and feasible algae removal and provides good germplasm resources and technical support for the control of cyanobacterial bloom.
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影响因子：
6.6
作者：
Kaytee L. Pokrzywinski;A. Place;M. Warner;K. Coyne
通讯作者：
Kaytee L. Pokrzywinski;A. Place;M. Warner;K. Coyne
影响因子：
2.2
作者：
Mayali, X;Azam, F
通讯作者：
Azam, F
影响因子：
6.6
作者：
Su, Ran Qiang;Yang, Xiao Ru;Hong, Hua Sheng
通讯作者：
Hong, Hua Sheng
影响因子：
3.4
作者：
Namsaraev, Zorigto;Melnikova, Anna;Ivanov, Evgenii
通讯作者：
Ivanov, Evgenii
影响因子：
6.6
作者：
L. Zhou;T. Zheng;Xin Wang;J. Ye;Yun Tian;H. Hong
通讯作者：
L. Zhou;T. Zheng;Xin Wang;J. Ye;Yun Tian;H. Hong