M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis.
M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Inhibiting the Development of Endometriosis.
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M1 巨噬细胞衍生的纳米囊泡使 M2 巨噬细胞复极化，抑制子宫内膜异位症的发展
DOI：
10.3389/fimmu.2021.707784
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发表时间：
2021
影响因子：
7.3
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Li Q;Yuan M;Jiao X;Huang Y;Li J;Li D;Ji M;Wang G
子宫内膜异位症是一种常见的非恶性妇科疾病，影响10%-15%的育龄妇女，并导致多种症状，导致生活质量下降和巨大的社会负担。近几十年来，细胞外小泡(EVS)作为一种潜在的治疗工具受到了人们的关注，但EVS对子宫内膜异位症的疗效尚未见报道。因此，在本研究中，我们探讨了M1巨噬细胞来源的纳米囊泡(NV)治疗子宫内膜异位症的可行性。采用连续挤压法制备M1NV。采用共培养方法观察子宫内膜异位症(EM-ESCs)患者在位内膜基质细胞(ESCs)在体内膜间质细胞(ESCs)在体管形成和迁移/侵袭的变化。建立子宫内膜异位症小鼠模型，用磷酸盐缓冲液、M0Nvs或M1Nvs治疗，以评价M1NV治疗子宫内膜异位症的疗效和安全性。M1Nvs直接或间接抑制EM-ESCs的迁移和侵袭，减少管状细胞的形成。在小鼠模型中，M1Nvs通过M2巨噬细胞的重新编程抑制子宫内膜异位症的发展，而不会对器官造成损害。M1Nvs直接抑制子宫内膜异位症的发展，或通过将巨噬细胞从M2表型重新极化为M1表型。因此，M1Nvs的应用可能是治疗子宫内膜异位症的一种新方法。
Endometriosis is a common nonmalignant gynecological disorder that affects 10–15% women of reproductive age and causes several symptoms that result in decreased quality of life and a huge social burden. In recent decades, extracellular vesicles (EVs) have gained attention as a potential therapeutic tool; however, the therapeutic effects of EVs against endometriosis have not been reported. Accordingly, in this study, we investigated the feasibility of nanovesicles (NVs) derived from M1 macrophages (M1NVs) in treating endometriosis. M1NVs were prepared by serial extrusion. Co-culture assays were performed to investigate changes in tube formation and migration/invasion of eutopic endometrial stroma cells (ESCs) obtained from patients with endometriosis (EM-ESCs). A mouse model of endometriosis was established, and mice were treated with phosphate-buffered saline, M0NVs, or M1NVs to evaluate the efficacy and safety of M1NV for treating endometriosis. M1NVs directly or indirectly inhibited the migration and invasion of EM-ESCs and reduced tube formation. In the mouse model, M1NVs suppressed the development of endometriosis through reprogramming of M2 macrophages, without causing damage to the organs. M1NVs inhibit the development of endometriosis directly, or through repolarizing macrophages from M2 to M1 phenotype. Hence, administration of M1NVs may represent a novel method for the treatment of endometriosis.
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影响因子：
7.3
作者：
Capobianco A;Rovere-Querini P
通讯作者：
Rovere-Querini P
影响因子：
30.5
作者：
Marar, Carolyn;Starich, Bartholomew;Wirtz, Denis
通讯作者：
Wirtz, Denis
M2巨噬细胞衍生的外泌体促进结肠癌细胞迁移和侵袭
影响因子：
11.2
作者：
Lan, Jingqin;Sun, Li;Wang, Guihua
通讯作者：
Wang, Guihua
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影响因子：
29.4
作者：
Ding, Jingjing;Lu, Guihong;Xie, Hai-Yan
通讯作者：
Xie, Hai-Yan
影响因子：
9.8
作者：
Groothuis, P. G.;Nap, A. W.;Gruemmer, R.
通讯作者：
Gruemmer, R.