Direct and label-free detection of cholic acid based on molecularly imprinted photonic hydrogels
Direct and label-free detection of cholic acid based on molecularly imprinted photonic hydrogels
复制标题
基于分子印迹光子水凝胶的胆酸直接无标记检测
DOI：
10.1039/b811189h
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发表时间：
2008-12
影响因子：
--
通讯作者：
Hu, XB
中科院分区：
文献类型：
--
作者：
Liu, J;Tao, CA;Li, GT;Shen, DZ;Yang, CD;Wu, Z;Li, Y;Hu, XB
在这里，我们报告的设计和制备一种新的自我报告传感器胆酸，一个重要的生物化合物在肝细胞中产生。胆酸的传统检测方法大多依赖于分析设备，并且要么耗时，要么需要衍生化过程。在这项工作中，分子印迹光子水凝胶（IPHs）的基础上的一种新的方法进行了描述，通过该方法可以实现直接，灵敏和无标记的检测胆酸没有任何衍生化处理和昂贵的仪器。将胶体晶体模板法与分子印迹技术相结合，制备了一种独特的三维有序多孔水凝胶，该水凝胶显示出对胆酸浓度响应的光学变化。由于其特殊的分层多孔结构，其中包括3D有序的互连大孔阵列与来自分子印迹的纳米腔，所形成的光子水凝胶允许快速和超灵敏的检测目标分析物。互连的大孔有利于分析物在水凝胶中的快速运输，而分布在薄水凝胶壁中的纳米腔的固有高亲和力允许IPH以高特异性识别分析物。
Here we report the design and preparation of a novel self-reporting sensor for cholic acid, an important biological compound produced in hepatocytes. Traditional detection methods of cholic acid are mostly dependent on analytical equipment, and are either time-consuming or require a derivatization process. In this work, a new approach based on molecularly imprinted photonic hydrogels (IPHs) is described, by which direct, sensitive and label-free detection of cholic acid can be achieved without any derivatization treatment and expensive instruments. The unique 3D ordered porous hydrogels that reveal optical changes in responsive to cholic acid concentration were prepared by combining colloidal crystal templating with the molecular imprinting technique. Due to their special hierarchical porous structure, which consists of 3D-ordered interconnected macroporous arrays with nanocavities derived from molecular imprinting, the formed photonic hydrogels allow rapid and ultrasensitive detection of the target analyte. The interconnected macropores are favorable for the rapid transport of the analyte in the hydrogel, while the inherent high affinity of nanocavities distributed in thin hydrogel walls allows IPHs to recognize the analyte with high specificity.
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DOI：
10.1039/b004580m
发表时间：
2000
期刊：
The Analyst
影响因子：
--
作者：
T. Kato;K. Yano;K. Ikebukuro;I. Karube
通讯作者：
T. Kato;K. Yano;K. Ikebukuro;I. Karube
DOI：
--
发表时间：
2017
期刊：
--
影响因子：
--
作者：
T. Barder;Firn-Harold N. Wells;H. Gerhard;Fuchs;G. Rooney
通讯作者：
T. Barder;Firn-Harold N. Wells;H. Gerhard;Fuchs;G. Rooney
影响因子：
7.4
作者：
Ben-Moshe, Matti;Alexeev, Vladimir L.;Asher, Sanford A.
通讯作者：
Asher, Sanford A.
影响因子：
29.4
作者：
Lee, YJ;Braun, PV
通讯作者：
Braun, PV
DOI：
10.1016/j.jchromb.2007.09.031
发表时间：
2007-12-01
影响因子：
3
作者：
Ye, Lian;Liu, Suya;Ding, Min
通讯作者：
Ding, Min