Eosinophil protein in airway macrophages: a novel biomarker of eosinophilic inflammation in patients with asthma.
Eosinophil protein in airway macrophages: a novel biomarker of eosinophilic inflammation in patients with asthma.
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DOI：
10.1016/j.jaci.2010.03.026
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发表时间：
2010-07
影响因子：
14.2
通讯作者：
Brightling, Christopher E.
中科院分区：
文献类型：
--
作者：
Kulkarni, Neeta S.;Hollins, Fay;Sutcliffe, Amanda;Saunders, Ruth;Shah, Sachil;Siddiqui, Salman;Gupta, Sumit;Haldar, Pranab;Green, Ruth;Pavord, Ian;Wardlaw, Andrew;Brightling, Christopher E.
嗜酸性粒细胞性哮喘在哮喘严重程度中很常见。然而，在中重度疾病患者中，痰嗜酸性粒细胞的缺失不能区分由糖皮质激素控制的嗜酸性粒细胞炎症与非嗜酸性粒细胞炎症的哮喘患者。我们试图开发一种方法来量化气道巨噬细胞中的嗜酸性粒细胞蛋白，作为嗜酸性气道炎症的一种新的生物标志物。通过流式细胞术、免疫荧光和摄入凋亡的嗜酸性粒细胞后细胞质色相的变化来评估气道巨噬细胞中的嗜酸性粒细胞蛋白。通过图像分析(1)轻至重度哮喘患者、非哮喘性嗜酸性支气管炎患者和健康对照者，评估染色痰液中红色区域的气道巨噬细胞中位数百分比；(2)强的松龙治疗后嗜酸性严重哮喘患者；(3)皮质类固醇停药前患有非嗜酸性哮喘的受试者。气道巨噬细胞中检测到嗜酸性细胞蛋白，摄入凋亡的嗜酸性细胞后细胞质呈红色。中重度哮喘患者的气道巨噬细胞百分比与轻度哮喘患者和健康对照者相比呈红色区域增加，痰嗜酸性粒细胞增多或无痰嗜酸性粒细胞增多者相似，皮质类固醇治疗后气道巨噬细胞百分比增加。在没有嗜酸性痰的哮喘受试者中，在皮质类固醇停药后，有嗜酸性痰的受试者与没有嗜酸性痰的受试者相比，气道巨噬细胞红区百分比增加。在气道巨噬细胞中可以可靠地测量嗜酸性粒细胞蛋白。结合痰嗜酸性粒细胞，巨噬细胞嗜酸性粒细胞蛋白含量可能进一步确定哮喘表型，并为指导治疗提供额外的工具。
Noneosinophilic asthma is common across asthma severities. However, in patients with moderate-to-severe disease, the absence of sputum eosinophilia cannot distinguish between asthmatic subjects with eosinophilic inflammation controlled by corticosteroids versus those in whom eosinophilic inflammation is not a component of the disease. We sought to develop a method to quantify eosinophil proteins in airway macrophages as a novel biomarker of eosinophilic airway inflammation. Eosinophil proteins in airway macrophages were assessed by means of flow cytometry, immunofluorescence, and cytoplasmic hue change after ingestion of apoptotic eosinophils. Airway macrophage median percentage of red-hued area in stained sputum cytospin preparations was assessed by means of image analysis from (1) subjects with mild-to-severe asthma, subjects with nonasthmatic eosinophilic bronchitis, and healthy control subjects; (2) subjects with eosinophilic severe asthma after treatment with prednisolone; and (3) subject with noneosinophilic asthma before corticosteroid withdrawal. Eosinophil proteins were detected in airway macrophages, and cytoplasmic red hue increased after ingestion of apoptotic eosinophils. Airway macrophage percentage red-hued area was increased in subjects with moderate-to-severe asthma compared with that seen in subjects with mild asthma and healthy control subjects, was similar in those with or without a sputum eosinophilia, and was increased after corticosteroid therapy. In asthmatic subjects without sputum eosinophilia, the airway macrophage percentage red-hued area was increased in subjects who did versus those who did not have sputum eosinophilia after corticosteroid withdrawal. Eosinophil proteins can be reliably measured in airway macrophages. In combination with sputum eosinophilia, the macrophage eosinophil protein content might further define the asthma phenotype and provide an additional tool to direct therapy.
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影响因子：
2.2
作者：
Day, C. E.;Zhang, S. D.;Guillen, C.
通讯作者：
Guillen, C.
影响因子：
6.9
作者：
Simpson, JL;Scott, R;Gibson, PG
通讯作者：
Gibson, PG
影响因子：
9.6
作者：
Gupta, Sumit;Siddiqui, Salman;Brightling, Christopher E.
通讯作者：
Brightling, Christopher E.
DOI：
10.1164/ajrccm.161.1.9809100
发表时间：
2000-01-01
影响因子：
24.7
作者：
Jatakanon, A;Lim, S;Barnes, PJ
通讯作者：
Barnes, PJ
影响因子：
9.6
作者：
Brightling, CE
通讯作者：
Brightling, CE