Mechanical stress regulates autophagic flux to affect apoptosis after spinal cord injury.
Mechanical stress regulates autophagic flux to affect apoptosis after spinal cord injury.
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机械应激调节自噬流影响脊髓损伤后细胞凋亡
DOI：
10.1111/jcmm.15863
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发表时间：
2020-11
影响因子：
5.3
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Zhang X;Jing Y;Qin C;Liu C;Yang D;Gao F;Yang M;Du L;Li J
Increased mechanical stress after spinal cord injury (SCI) expands the scope of nerve tissue damage and exacerbates nerve function defects. Surgical decompression after SCI is a conventional therapeutic strategy and has been proven to have neuroprotective effects. However, the mechanisms of the interaction between mechanical stress and neurons are currently unknown. In this study, we monitored intramedullary pressure (IMP) and investigated the therapeutic benefit of decompression (including durotomy and piotomy) after injury and its underlying mechanisms in SCI. We found that decreased IMP promotes the generation and degradation of LC3 II, promotes the degradation of p62 and enhances autophagic flux to alleviate apoptosis. The lysosomal dysfunction was reduced after decompression. Piotomy was better than durotomy for the histological repair of spinal cord tissue after SCI. However, the autophagy‐lysosomal pathway inhibitor chloroquine (CQ) partially reversed the apoptosis inhibition caused by piotomy after SCI, and the structural damage was also aggravated after CQ administration. An antibody microarray analysis showed that decompression may reverse the up‐regulated abundance of p‐PI3K, p‐AKT and p‐mTOR caused by SCI. Our findings may contribute to a better understanding of the mechanism of decompression and the effects of mechanical stress on autophagy after SCI.
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影响因子：
3
作者：
Fehlings, Michael G.;Rabin, Doron;Aarabi, Bizhan
通讯作者：
Aarabi, Bizhan
影响因子：
4.2
作者：
Dvorak, Marcel F.;Noonan, Vanessa K.;Fehlings, Michael G.
通讯作者：
Fehlings, Michael G.
影响因子：
4.2
作者：
Khaing, Zin Z.;Cates, Lindsay N.;Hofstetter, Christoph P.
通讯作者：
Hofstetter, Christoph P.
自噬诱导可稳定微管并促进脊髓损伤后轴突再生。
DOI：
10.1073/pnas.1611282113
发表时间：
2016-10-04
影响因子：
11.1
作者：
He, Miao;Ding, Yuetong;Luo, Zhen-Ge
通讯作者：
Luo, Zhen-Ge
影响因子：
3.4
作者：
Huang, W. L.;George, K. J.;Priestley, J. V.
通讯作者：
Priestley, J. V.