Preinvasive colorectal lesion transcriptomes correlate with endoscopic morphology (polypoid vs. nonpolypoid).
Preinvasive colorectal lesion transcriptomes correlate with endoscopic morphology (polypoid vs. nonpolypoid).
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DOI：
10.1002/emmm.201100141
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发表时间：
2011-06
影响因子：
11.1
通讯作者：
Marra, Giancarlo
中科院分区：
文献类型：
--
作者：
Cattaneo, Elisa;Laczko, Endre;Buffoli, Federico;Zorzi, Fausto;Bianco, Maria Antonia;Menigatti, Mirco;Bartosova, Zdena;Haider, Ritva;Helmchen, Birgit;Sabates-Bellver, Jacob;Tiwari, Amit;Jiricny, Josef;Marra, Giancarlo
改进的结肠镜检查发现了过去经常被遗漏的癌前病变，今天检测到的∼中有30%的人有从轻微隆起到凹陷的非息肉形态。为了从分子上描述这些病变，我们评估了42个癌前病变(25个轻微升高的非息肉体和17个带蒂息肉样)中23,768个基因的转录，每一个都有相应的正常黏膜样本。非息肉体和息肉体的形态可以解释样本之间的大部分基因表达差异；组织学、异型增生的大小和程度也与特定的模式有关。息肉样病变的表达变化经常影响细胞周期途径，而非息肉样病变的细胞生存失调占主导地位。与息肉样病变相比，后者的表达变化也更少、更不明显。这一趋势的一个范例是通过TMIGD1mRNA和蛋白的正常、非息肉体、息肉体和癌基因序列的进行性丢失。这一发现，以及TMIGD1蛋白在组织和细胞系中的表达模式，表明TMIGD1可能与肠道细胞分化有关。我们的结论是，在轻微升高的非息肉性癌前病变中，分子调节失调可能比在经典性腺瘤性息肉中观察到的稍微轻一些。
Improved colonoscopy is revealing precancerous lesions that were frequently missed in the past, and ∼30% of those detected today have nonpolypoid morphologies ranging from slightly raised to depressed. To characterize these lesions molecularly, we assessed transcription of 23,768 genes in 42 precancerous lesions (25 slightly elevated nonpolypoid and 17 pedunculated polypoid), each with corresponding samples of normal mucosa. Nonpolypoid versus polypoid morphology explained most gene expression variance among samples; histology, size, and degree of dysplasia were also linked to specific patterns. Expression changes in polypoid lesions frequently affected cell-cycling pathways, whereas cell-survival dysregulation predominated in nonpolypoid lesions. The latter also displayed fewer and less dramatic expression changes than polypoid lesions. Paradigmatic of this trend was progressive loss through the normal > nonpolypoid > polypoid > cancer sequence of TMIGD1 mRNA and protein. This finding, along with TMIGD1 protein expression patterns in tissues and cell lines, suggests that TMIGD1 might be associated with intestinal-cell differentiation. We conclude that molecular dysregulation in slightly elevated, nonpolypoid, precancerous colorectal lesions may be somewhat less severe than that observed in classic adenomatous polyps.
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影响因子：
37.3
作者：
Menigatti M;Cattaneo E;Sabates-Bellver J;Ilinsky VV;Went P;Buffoli F;Marquez VE;Jiricny J;Marra G
通讯作者：
Marra G
影响因子：
7.7
作者：
Lambert, Rene;Kudo, Shin ei;Triadafilopoulos, George
通讯作者：
Triadafilopoulos, George
影响因子：
120.7
作者：
Soetikno, Roy M.;Kaltenbach, Tonya;Friedland, Shai
通讯作者：
Friedland, Shai
基因贡献的稳定性和对微阵列数据的多元分析中异常值的识别。
影响因子：
3
作者：
Baty, Florent;Jaeger, Daniel;Preiswerk, Frank;Schumacher, Martin M.;Brutsche, Martin H.
通讯作者：
Brutsche, Martin H.
影响因子：
12.6
作者：
O'Brien, Michael J.;Winawer, Sidney J.;Schapiro, Melvin
通讯作者：
Schapiro, Melvin