A novel role for the TIR domain in association with pathogen-derived elicitors.
A novel role for the TIR domain in association with pathogen-derived elicitors.
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DOI：
10.1371/journal.pbio.0050068
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发表时间：
2007-03
期刊：
影响因子：
9.8
通讯作者：
Dinesh-Kumar SP
中科院分区：
文献类型：
--
作者：
Burch-Smith TM;Schiff M;Caplan JL;Tsao J;Czymmek K;Dinesh-Kumar SP
植物天然免疫是由抗性蛋白介导的，抗性蛋白与具有类似功能的动物分子具有惊人的相似性。烟草N是TIR-NB-LRR R基因，其赋予对烟草花叶病毒的抗性，特别是p50解旋酶结构域。一个有趣的问题是植物R蛋白如何识别病原体衍生的无毒（Avr）诱导蛋白的存在。我们已经使用生化细胞分数和免疫沉淀，除了共聚焦荧光显微镜的活组织检查N和p50之间的关联。令人惊讶的是，N和p50都是细胞质和核蛋白，N的核定位是其功能所必需的。我们还证明了在植物N和p50之间的关联。此外，我们发现N的TIR结构域对于这种关联是至关重要的，事实上，它可以单独与p50相关联。我们的研究结果不同于目前的植物先天免疫模型，该模型提出检测仅通过这些分子的LRR结构域介导。我们提出的数据支持一个复杂的过程中病原体激发子识别R蛋白涉及多个亚细胞区室和多个蛋白质复合物的形成。每年，高达10%的世界农业产量因各种病原体（包括细菌、真菌、线虫和病毒）引起的害虫和疾病而损失。近一个世纪以来，科学家们已经了解到植物具有自己的免疫系统，可以积极地与病原体接触并预防许多感染。植物免疫系统的一个方面由基因对基因假说定义：植物抗性（R）基因编码特异性识别并保护免受携带相应无毒（Avr）基因的病原体或病原体菌株的蛋白质。在烟草及其近缘植物中，N抗性蛋白赋予对烟草花叶病毒（TMV）感染的抗性。我们已经使用N和TMV激发子p50来研究基因对基因抗性的机制。我们发现，N和p50在植物细胞的细胞质和细胞核中相关联，这种关联是由N的TIR结构域介导的，其结构类似于动物先天免疫分子。我们的研究结果为R蛋白如何识别病原体Avr蛋白提供了新的见解，并应有助于提高作物产量的长期努力。植物抗病基因如何识别同源病原菌？这些作者剖析了抗性基因和病毒病原体的相互作用。
Plant innate immunity is mediated by Resistance (R) proteins, which bear a striking resemblance to animal molecules of similar function. Tobacco N is a TIR-NB-LRR R gene that confers resistance to Tobacco mosaic virus, specifically the p50 helicase domain. An intriguing question is how plant R proteins recognize the presence of pathogen-derived Avirulence (Avr) elicitor proteins. We have used biochemical cell fraction and immunoprecipitation in addition to confocal fluorescence microscopy of living tissue to examine the association between N and p50. Surprisingly, both N and p50 are cytoplasmic and nuclear proteins, and N's nuclear localization is required for its function. We also demonstrate an in planta association between N and p50. Further, we show that N's TIR domain is critical for this association, and indeed, it alone can associate with p50. Our results differ from current models for plant innate immunity that propose detection is mediated solely through the LRR domains of these molecules. The data we present support an intricate process of pathogen elicitor recognition by R proteins involving multiple subcellular compartments and the formation of multiple protein complexes. Each year, up to 10% of world agricultural production is lost to pests and diseases caused by a variety of pathogens including bacteria, fungi, nematodes, and viruses. Scientists have understood for nearly a century that plants carry their own immune system that actively engages pathogens and prevents many infections. One aspect of the plant immune system is defined by the gene-for-gene hypothesis: a plant Resistance (R) gene encodes a protein that specifically recognizes and protects against one pathogen or strain of a pathogen carrying a corresponding Avirulence (Avr) gene. In tobacco and its relatives, the N resistance protein confers resistance to infection by the Tobacco mosaic virus (TMV). We have used N, and the TMV elicitor, p50, to investigate the mechanism of gene-for-gene resistance. We show that N and p50 associate in the cytoplasm and nucleus of plant cells and that this association is mediated by N's TIR domain, which is structurally similar to animal innate immunity molecules. Our findings provide novel insight into how R proteins recognize pathogen Avr proteins, and should help in long-term efforts to enhance crop yield. How do plant resistance gene recognize the cognate pathogen? These authors dissect the interaction of a resistance gene and a viral pathogen.
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影响因子：
11.6
作者：
Bieri, S;Mauch, S;Schulze-Lefert, P
通讯作者：
Schulze-Lefert, P
影响因子：
7.2
作者：
Erickson, FL;Holzberg, S;Baker, B
通讯作者：
Baker, B
影响因子：
11.4
作者：
Jia, Y;McAdams, SA;Valent, B
通讯作者：
Valent, B
DOI：
10.1073/pnas.97.26.14789
发表时间：
2000-12-19
影响因子：
11.1
作者：
Dinesh-Kumar, SP;Tham, WH;Baker, BJ
通讯作者：
Baker, BJ
影响因子：
11.6
作者：
Leister, RT;Dahlbeck, D;Staskawicz, BJ
通讯作者：
Staskawicz, BJ