Recognition of tissue- and subtype-specific modulation of angiotensin II receptors using antibodies against AT1 and AT2 receptors.
Recognition of tissue- and subtype-specific modulation of angiotensin II receptors using antibodies against AT1 and AT2 receptors.
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使用针对 AT1 和 AT2 受体的抗体识别血管紧张素 II 受体的组织和亚型特异性调节。
作者：
H. Rakugi;A. Okamura;K. Kamide;M. Ohishi;H. Sasamura;R. Morishita;J. Higaki;T. Ogihara
制备了针对人血管紧张素II 1型（AT1）和2型（AT2）受体合成肽的多克隆抗体。合成的肽与AT1受体的氨基酸15-24和AT2受体的氨基酸241-253相对应。转染小鼠AT1b受体、人血管平滑肌细胞和大鼠外周组织表达质粒的COS-7细胞匀浆膜的Western blot分析显示，AT1受体抗体的主要条带为MW 44,000。转染人AT2受体表达质粒的COS-7细胞匀浆显示AT2受体抗体条带为MW 44,000。大鼠肾上腺髓质组织匀浆和人嗜铬细胞瘤组织匀浆中存在AT2受体抗体的mw52000大条带和mw44000小条带。AT1受体的表达顺序为：大鼠主动脉> >肺、肾、脾、肾上腺皮质>肾上腺髓质、心脏。AT2受体的表达顺序为：大鼠肾上腺髓质>皮层、肾脏、心脏。用AT1受体拮抗剂CS866和血管紧张素转换酶抑制剂替莫普利治疗3天，抑制了大鼠肾上腺皮质的AT1受体表达，但在心脏或肾上腺髓质中没有。AT2的表达不受这些药物治疗的影响。这些结果表明，新开发的AT1和AT2受体抗体有助于阐明亚型特异性受体表达的调控，并且肾上腺组织中的AT1受体而不是AT2受体受血管紧张素II的调控。
Polyclonal antibodies have been prepared against synthetic peptides of human angiotensin II type-1 (AT1) and type-2 (AT2) receptors. Synthetic peptides corresponded to amino acids 15-24 of AT1 receptor and amino acids 241-253 of AT2 receptor. Western blot analysis of membranes from cell homogenates of COS-7 cells transfected with expression plasmids for mouse AT1b receptor, human vascular smooth muscle cells, and rat peripheral tissue demonstrated a major band of MW 44,000 with AT1 receptor antibody. Cell homogenates of COS-7 cells transfected with expression plasmids for human AT2 receptor showed a band of MW 44,000 with AT2 receptor antibody. Tissue homogenate of rat adrenal medulla and human pheochromocytoma presented a major band of MW 52,000 and a minor band of MW 44,000 with AT2 receptor antibody. AT1 receptor expression was in the following order: rat aorta > > lung, kidney, spleen, adrenal cortex > adrenal medulla, heart. Expression of AT2 receptor was in the following order: rat adrenal medulla > cortex, kidney, heart. Three-day treatment with CS866, an AT1 receptor antagonist, and temocapril, an angiotensin-converting enzyme inhibitor, suppressed AT1 receptor expression in the rat adrenal cortex, but not in the heart or adrenal medulla. AT2 expression was not affected by treatment with these drugs. These results suggest that newly developed antibodies for AT1 and AT2 receptors are useful in elucidating the regulation of subtype-specific receptor expression, and that AT1 but not AT2 receptors in adrenal tissue are regulated by angiotensin II.
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血管紧张素 II 与大鼠肾上腺膜结合位点的共价交联。
DOI：
10.1073/pnas.79.12.3739
发表时间：
1982
影响因子：
11.1
作者：
Paglin,S;Jamieson,JD
通讯作者：
Jamieson,JD
血管紧张素 II 1 型 (AT1) 受体多克隆抗肽抗体的表征。
DOI：
10.1016/0006-291x(92)90551-u
发表时间：
1992
影响因子：
3.1
作者：
Zelezna,B;Richards,EM;Tang,W;Lu,D;Sumners,C;Raizada,MK
通讯作者：
Raizada,MK
影响因子：
--
作者：
PAXTON, WG;RUNGE, M;BERNSTEIN, KE
通讯作者：
BERNSTEIN, KE
人类 1 型血管紧张素 II 受体的遗传分析。
DOI：
10.1210/mend.6.7.1508224
发表时间：
1992
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Curnow,KM;Pascoe,L;White,PC
通讯作者：
White,PC
大鼠脑血管紧张素 II 受体的溶解和部分表征。
影响因子：
4.7
作者：
Siemens,IR;Swanson,GN;Fluharty,SJ;Harding,JW
通讯作者：
Harding,JW