Characterization of PrpC from Bacillus subtilis, a Member of the PPM Phosphatase Family
Characterization of PrpC from Bacillus subtilis, a Member of the PPM Phosphatase Family
复制标题
PPM 磷酸酶家族成员枯草芽孢杆菌中 PrpC 的表征
作者：
M. Obuchowski;E. Madec;D. Delattre;G. Boël;A. Iwanicki;D. Foulger;S. Séror
摘要我们克隆了YloO基因，并纯化了其产物的一种His标记形式，即推测的蛋白磷酸酶YloO，我们现在将其命名为PrPC。这在序列和预测的二级结构上与人类蛋白磷酸酶PP2C非常相似，PPM家族的成员之一。PrPC在体外对合成底物对硝基苯酚磷酸和内源性枯草芽孢杆菌蛋白具有磷酸酶活性。PrkC和prpC基因在染色体上相邻，PrkC的磷酸化形式是PrPC的底物。这些发现表明PrkC和PrPC在体内可能是成对发挥作用的。
ABSTRACT We cloned the yloO gene and purified a His-tagged form of its product, the putative protein phosphatase YloO, which we now designate PrpC. This closely resembles the human protein phosphatase PP2C, a member of the PPM family, in sequence and predicted secondary structure. PrpC has phosphatase activity in vitro against a synthetic substrate, p-nitrophenol phosphate, and endogenousBacillus subtilis proteins. The prkC andprpC genes are adjacent on the chromosome, and the phosphorylated form of PrkC is a substrate for PrpC. These findings suggest that PrkC and PrpC may function as a couple in vivo.
影响因子：
10.5
作者：
Xiaofeng Yang;Choong-Min Kang;M. S. Brody;C. Price
通讯作者：
Xiaofeng Yang;Choong-Min Kang;M. S. Brody;C. Price
DOI：
10.2741/kennelly
发表时间：
1999-03-15
期刊：
Frontiers in Bioscience
影响因子：
--
作者：
Kennelly, Peter J.;Potts, Malcolm
通讯作者：
Potts, Malcolm