Increased apoptosis, changes in intracellular Ca2+, and functional alterations in lymphocytes and macrophages after in vitro exposure to static magnetic field.
Increased apoptosis, changes in intracellular Ca2+, and functional alterations in lymphocytes and macrophages after in vitro exposure to static magnetic field.
复制标题
体外暴露于静磁场后，细胞凋亡增加、细胞内 Ca2+ 变化以及淋巴细胞和巨噬细胞功能改变。
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
K. Krzystyniak
中科院分区：
文献类型：
--
作者：
D. Flipo;M. Fournier;C. Benquet;Philippe P Roux;C. Le Boulaire;C. Pinsky;F. LaBella;K. Krzystyniak
在极低频低能量脉冲电磁场（ELF-EMF）中，与电磁相关的细胞功能改变得到了充分的证明。在体外研究了静磁场（SMF）对C57BI/6小鼠巨噬细胞、脾脏淋巴细胞和胸腺细胞免疫参数的影响。细胞在37℃，5% CO2, 250-1500 G SMF条件下体外培养24 h。暴露于SMF导致荧光乳胶微球的吞噬作用减少，这伴随着巨噬细胞细胞内Ca2+水平的增加。暴露于SMF降低了淋巴细胞的有丝分裂反应，这是通过将[3H]胸腺嘧啶掺入细胞来确定的。这与豆豆蛋白a刺激淋巴细胞中Ca2+内流增加有关。此外，通过流式细胞术检测，暴露于SMF可显著增加胸腺细胞的凋亡。总的来说，体外暴露于250-1500 G SMF的免疫活性细胞改变了C57BI/6小鼠巨噬细胞、胸腺细胞和脾脏淋巴细胞的几个功能参数。
Electromagnetic-related alteration of cellular functions is well documented for extremely low-frequency low-energy pulsing electromagnetic fields (ELF-EMF). In this study we examined the in vitro effects of static magnetic fields (SMF) on the cellular immune parameters of the C57BI/6 murine macrophages, spleen lymphocytes, and thymic cells. The cells were exposed in vitro for 24 h at 37 degrees C, 5% CO2, to 250-1500 G SMF. Exposure to the SMF resulted in the decreased phagocytic uptake of fluorescent latex microspheres, which was accompanied by an increased intracellular Ca2+ level in macrophages. Exposure to SMF decreased mitogenic responses in lymphocytes, as determined by incorporation of [3H]thymidine into the cells. This was associated with the increased Ca2+ influx in concanavalin A-stimulated lymphocytes. Furthermore, exposure to SMF produced markedly increased apoptosis of thymic cells, as determined by flow cytometry. Overall, in vitro exposure of immunocompetent cells to 250-1500 G SMF altered several functional parameters of C57BI/6 murine macrophages, thymocytes, and spleen lymphocytes.
氧化剂对淋巴细胞生长的差异调节作用：胞质 Ca 2 的变化。
影响因子：
3.8
作者：
Duncan,DD;Lawrence,DA
通讯作者：
Lawrence,DA
肺巨噬细胞摄取乳胶颗粒：钙的作用。
DOI：
10.1152/ajpcell.1983.245.3.c227
发表时间：
1983
期刊：
The American journal of physiology
影响因子：
--
作者：
Parod,RJ;Brain,JD
通讯作者：
Brain,JD